We narrowed to 7,456 results for: Ski;

1. pLdCN

   Plasmid

   #84290

   Purpose

   Express gRNA and Cas9 in Leishmania with Neomycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-7 aSyn C141

   Plasmid

   #108866

   Purpose

   Expresses human WT alpha synuclein with a C-terminal cysteine

   Depositor

   Gabriele Kaminski Schierle

   Article

   Stephens et al bioRxiv 2022.10.04.510646

   Insert

   alpha synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C141 mutation to allow C-terminal maleimide dye l…

   Promoter

   T7

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Designed VEGF-binding scFv G6des13

   Plasmid

   #110213

   Purpose

   Yeast Surface Display of G6des13

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des13
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

   Promoter

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Designed VEGF-binding scFv G6des1

   Plasmid

   #110212

   Purpose

   Yeast Surface Display of G6des1

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des1
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

   Promoter

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HEL binding scFv D44.1

   Plasmid

   #111718

   Purpose

   Yeast Surface Display of D44.1 as a reference starting point for design

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   D44.1
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Designed HEL-binding scFv D44.1des

   Plasmid

   #111719

   Purpose

   Yeast Surface Display of D44.1des

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   D44.1 des
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…

   Promoter

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cyclin D1 shRNA-B

   Plasmid

   #32494

   Depositor

   Peter Sicinski

   Article

   Jirawatnotai et al Nature. 2011 Jun 8;474(7350):230-4. doi:

   Insert

   Cyclin D1 (CCND1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

8. CAG-Cas9-T2A-EGFP-ires-puro

   Plasmid

   #78311

   Purpose

   Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

   Depositor

   Timo Otonkoski

   Article

   Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

   Insert

   WT SpCas9-T2A-EGFP-ires-puromycin resistance
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

    Plasmid

    #175572

    Purpose

    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAC3-tag-N

    Plasmid

    #185482

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/UltralD

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Type

    Empty backbone

    Use

    Tags

    StrepIII-HA-UltraID

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC3-tag-C

    Plasmid

    #185481

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/UltralD

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Type

    Empty backbone

    Use

    Tags

    StrepIII-HA-UltraID

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAC_C_RON

    Plasmid

    #187783

    Purpose

    MAC-tagged gene expression of human RON

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    RON
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLys-M1 backbone

    Plasmid

    #109386

    Purpose

    Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pECFP18aaEYFP

    Plasmid

    #109330

    Purpose

    ECFP-EYFP FRET positive control

    Depositor

    Gabriele Kaminski Schierle

    Article

    Kaminski et al Methods Mol Biol. 2014;1076:445-54. doi:

    Insert

    ECFP-EYFP FRET positive control
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ECFP linked to EYFP with 18 aa linker GLRSRAQASNS…

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC_C_DUX4

    Plasmid

    #187799

    Purpose

    MAC-tagged gene expression of human DUX4

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pD864-LacZ

    Plasmid

    #109380

    Purpose

    Rhamnose inducible LacZ expression. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pRHAM-LacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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