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Plasmid#111718PurposeYeast Surface Display of D44.1 as a reference starting point for designDepositorInsertD44.1
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Designed HEL-binding scFv D44.1des
Plasmid#111719PurposeYeast Surface Display of D44.1desDepositorInsertD44.1 des
UseTagscmyc tagExpressionYeastMutationL Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…PromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cyclin D1 shRNA-B
Plasmid#32494DepositorInsertCyclin D1 (CCND1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterCAGAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)
Plasmid#175572PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)
Plasmid#175571PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)
Plasmid#175574PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)
Plasmid#175573PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC3-tag-N
Plasmid#185482PurposeGateway compatible destination vectors containing StrepIII/HA/UltralDDepositorTypeEmpty backboneUseTagsStrepIII-HA-UltraIDExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC3-tag-C
Plasmid#185481PurposeGateway compatible destination vectors containing StrepIII/HA/UltralDDepositorTypeEmpty backboneUseTagsStrepIII-HA-UltraIDExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_RON
Plasmid#187783PurposeMAC-tagged gene expression of human RONDepositorInsertRON
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECFP18aaEYFP
Plasmid#109330PurposeECFP-EYFP FRET positive controlDepositorInsertECFP-EYFP FRET positive control
UseTagsExpressionMammalianMutationECFP linked to EYFP with 18 aa linker GLRSRAQASNS…PromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_DUX4
Plasmid#187799PurposeMAC-tagged gene expression of human DUX4DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1 backbone
Plasmid#109386PurposeEmpty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-Cas9-H2B-mCherry
Plasmid#175570PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationXbaI site added upstream of U6 promotor. G to C …PromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_ROS1
Plasmid#187766PurposeMAC-tagged gene expression of human ROS1DepositorInsertROS1 (ROS1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-Lux
Plasmid#109383PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coliDepositorInsertpARABAD-LUX operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_MUSK
Plasmid#187781PurposeMAC-tagged gene expression of human MUSKDepositorInsertMUSK (MUSK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1
Plasmid#109382PurposeArabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/TagRFP-LL5b
Plasmid#112828Purposetransient/stable overexpressionDepositorInsertLL5b (Phldb2 Mouse)
UseTagsTagRFPExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only