Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,951 results for: T7

Clear keywords
Showing: 181 - 200 of 10951 results

  5. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

    Enlarge

  6. QUAS-5-T7-TetO-GFP_ColE1

    Plasmid
    #171660
    Purpose
    QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QUAS-5-T7-TetO-GFP ssra-T7 Stop
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171660-plasmid-map-sequence-id-339032

    Enlarge

  8. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Available Since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

    Enlarge

  10. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

    Enlarge

  16. pCDNA3 T7 Jip4 (1-272)

    Plasmid
    #58928
    Purpose
    JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6
    Depositor
    Roger Davis
    Article
    Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.
    Insert
    Mapk8ip4 (Spag9 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Mutation
    Jip4 (1-272aa)
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pCDNA3 T7 Jip4 (1-151)

    Plasmid
    #58926
    Purpose
    JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6
    Depositor
    Roger Davis
    Article
    Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.
    Insert
    Mapk8ip4 (Spag9 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Mutation
    Jip4 (1-151aa)
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pCDNA3 T7 Jip4 (1-212)

    Plasmid
    #58927
    Purpose
    JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6
    Depositor
    Roger Davis
    Article
    Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.
    Insert
    Mapk8ip4 (Spag9 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Mutation
    Jip4 (1-212aa)
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 181 - 200 of 10951 results