We narrowed to 945 results for: dcas9, grna

1. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMonAID_dCas9-PmCDA_Hyg_ALS

   Plasmid

   #91692

   Purpose

   Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A and H840A for dead Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA47_dCas9-GFP_AAN_4

   Plasmid

   #231415

   Purpose

   This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-X
   sgRNA-Y
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA47_dCas9-GFP_YES_i2

   Plasmid

   #231419

   Purpose

   This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-Y
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. (364) SB KRAB-SadCas9

   Plasmid

   #163022

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TET-On

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

   Plasmid

   #172985

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

   Plasmid

   #172984

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR)

   Plasmid

   #209773

   Purpose

   CRISPRi for STK4

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-KRAB, sgSTK4i (STK4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_dCas9-KRAB_sgSTK4i_#2 (PuroR)

   Plasmid

   #209774

   Purpose

   CRISPRi for STK4

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-KRAB, sgSTK4i (STK4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1

    Plasmid

    #172983

    Purpose

    CRISPRi for TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    sgTAOK2i (TAOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC154-dual-dCas9VP160-sgExpression

    Plasmid

    #48240

    Purpose

    Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC152-dual-dCas9VP64-sgExpression

    Plasmid

    #48238

    Purpose

    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA Tag, HA-Tag, and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Promoter

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC153-dual-dCas9VP96-sgExpression

    Plasmid

    #48239

    Purpose

    Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP96

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. (517) SB KRAB-SadCas9 U6-gStop

    Plasmid

    #163023

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9-J23109

    Plasmid

    #113151

    Purpose

    Plasmid constitutively expressing dCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A & H840A

    Promoter

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. peSpdCas9-J23109

    Plasmid

    #113153

    Purpose

    Plasmid constitutively expressing eSpdCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    eSpdCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A & K810A & H840A & K1003A & R…

    Promoter

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-Universal.Sth.dCas9

    Plasmid

    #171088

    Purpose

    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    

    Use

    Toxoplasma gondii expression

    Tags

    Expression

    Mutation

    Note: A S245F mutation is present but did not app…

    Promoter

    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV_dCas9-KRAB_sgSTK3i_#2 (PuroR)

    Plasmid

    #209772

    Purpose

    CRISPRi for STK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    dCas9-KRAB, sgSTK3i (STK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV_dCas9-KRAB_sgSTK3i_#1 (PuroR)

    Plasmid

    #209771

    Purpose

    CRISPRi for STK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    dCas9-KRAB, sgSTK3i (STK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC2-dual-dCas9VP48-sgExpression

    Plasmid

    #48236

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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