We narrowed to 5,545 results for: donor

1. AAVS1_Puro_hPGK1_EGFP_Donor

   Plasmid

   #178088

   Purpose

   HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human PGK1

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor_ShCAST_kanR

   Plasmid

   #127924

   Purpose

   Kanamycin resistance donor for ShCAST

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   KanR, R6K origin of replication
   

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. NPM1_mNeonGreen_Donor

   Plasmid

   #178093

   Purpose

   HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   NPM1-mNeonGreen HDR donor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PE7_AAVS1_knockin_HDR_donor

   Plasmid

   #223001

   Purpose

   HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMPRAdonor2

   Plasmid

   #49353

   Purpose

   minP-luc2 donor plasmid for MPRA library construction

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   luc2
   

   Use

   Luciferase

   Promoter

   minP

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBRC_double_attB_GFP_donor

   Plasmid

   #169692

   Purpose

   cargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)

   Depositor

   John Wang

   Article

   Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMPRAdonor1

   Plasmid

   #49352

   Purpose

   Promoterless luc2 donor plasmid for MPRA library construction

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   luc2
   

   Use

   Luciferase

   Promoter

   None

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMPRAdonor3

   Plasmid

   #49354

   Purpose

   pSV40mod-luc2 donor plasmid for MPRA library construction

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   luc2
   

   Use

   Luciferase

   Promoter

   SV40mod

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1_Puro_hPGK1_EBFP_Donor

   Plasmid

   #178089

   Purpose

   HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EBFP (T65S/F145Y)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human PGK1

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDonor_sU6

    Plasmid

    #69351

    Purpose

    sgRNA scaffold and synthetic U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    sU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Promoter

    synthetic U6 promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. BB44_pmc.DonorR5.TS

    Plasmid

    #199223

    Purpose

    Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
    

    Use

    Gene targeting donor plasmid

    Expression

    Mammalian

    Promoter

    Human PGK1 gene promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDonor_mU6

    Plasmid

    #69350

    Purpose

    sgRNA scaffold and mouse U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    mU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Promoter

    mU6 promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AD59_pEP.DonorCLYBL.TS

    Plasmid

    #199227

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    

    Use

    Gene targeting donor plasmid

    Expression

    Mammalian

    Promoter

    Human PGK1 gene promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDonor_hU6

    Plasmid

    #69312

    Purpose

    sgRNA scaffold and human U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    hU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Promoter

    human U6 promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Donor_eGP2AP_RC

    Plasmid

    #133784

    Purpose

    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

    Depositor

    Sriram Kosuri

    Article

    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

    Type

    Empty backbone

    Tags

    eGFP-P2A-Puro

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDonor_AcCAST_kanR

    Plasmid

    #127925

    Purpose

    Kanamycin resistance donor for AcCAST

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    KanR
    

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATP1A1_plasmid_donor_RD

    Plasmid

    #86551

    Purpose

    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 (ATP1A1 Human)
    

    Use

    CRISPR and TALEN; Co-selection via hdr using ouab…

    Expression

    Mammalian

    Mutation

    Q118R, N129D

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AZ64_pE.DonorCLYBL.TS

    Plasmid

    #199228

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for EGFP reporter
    

    Use

    Gene targeting donor plasmid

    Expression

    Mammalian

    Promoter

    Human PGK1 gene promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Donor_ANKRD1_KO_Puro

    Plasmid

    #186667

    Purpose

    Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    ANKRD1 KO Puromycin (ANKRD1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Donor_YTHDF2_KO_Hygro

    Plasmid

    #186671

    Purpose

    Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Hygromycin (YTHDF2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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