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Showing: 181 - 200 of 14167 results

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pTRE-tight2-Tmem98-FLAG

Plasmid

#124506

Purpose

Expresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

tetO-CMV

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_HD (SPC right)

Plasmid

#126695

Purpose

right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC right

Use

TALEN

Tags

NLS, truncated N term and TAL, FokI

Expression

Mammalian

Mutation

R451P in SPC

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-TTlight-4xDQNAT

Plasmid

#128402

Purpose

Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Use

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

trc

Availability

Academic Institutions and Nonprofits only

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MR015-RBM20 Right TALEN

Plasmid

#108343

Purpose

Right TALEN for RBM20 with NN

Depositor

Bruce Conklin, Yuichiro Miyaoka

Article

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro-KrasG12D2B

Plasmid

#139786

Purpose

Lentiviral tet-inducible expression of KrasG12D (mouse)

Depositor

Joan Massague

Article

Su et al Nature. 2020 Jan;577(7791):566-571. doi:

Insert

KrasG12D (Kras Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G12D

Promoter

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV)

Plasmid

#140160

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight_A-CREB_PGK-tdTomato

Plasmid

#154264

Purpose

Dox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

dominant negative CREB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tight/tetO

Availability

Academic Institutions and Nonprofits only

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p3s-ND5-Right-G1397-N

Plasmid

#169213

Purpose

Express DdCBE for ND5 Point Mutation

Depositor

Jin-Soo Kim

Article

Lee et al Nat Commun. 2021 Feb 19;12(1):1190. doi:

Insert

DdCBE for ND5 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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APT3-PTEN (PTEN high)

Plasmid

#168006

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

PTEN-T3'PAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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CIp10 - YemVenus (OPT High)

Plasmid

#163122

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

YemVenus OPT High

Use

Candida albicans expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-Tight2-AkaLuc

Plasmid

#186186

Purpose

AAV vector packaging AkaLuciferase under the TRE promoter

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

Insert

AkaLuc luciferase

Use

AAV

Tags

Expression

Mutation

Promoter

TRE-Tight2

Availability

Academic Institutions and Nonprofits only

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HK02-LP Clone 2 light chain

Plasmid

#192179

Purpose

Clone 2 light chain (HK02)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 light chain (HK02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-TREtight-mTagBFP2-N2cG

Plasmid

#192838

Purpose

plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Insert

mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TREtight

Availability

Academic Institutions and Nonprofits only

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ET8-GPIba-high-6His

Plasmid

#180251

Purpose

High affinity mutant of human platelet membrane protein GP1b alpha

Depositor

Timothy Springer

Article

Jiang et al J Mol Biol. 2019 Mar 29;431(7):1380-1396

Insert

human GPIbalpha 1-290aa with mutation G233V and M239V (GP1BA Human)

Use

Tags

6His

Expression

Mammalian

Mutation

G233V and M239V

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-pMagHigh-FlpC

Plasmid

#180586

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 light chain

Plasmid

#175358

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 light chain

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 light chain

Use

Tags

N-terminal secretion signal peptide (MEAPAQLLFLLL…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLV-CMVtight-PLXNB2 iOE

Plasmid

#176849

Purpose

lentiviral vector for doxycycline-inducible PLXNB2 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

full length PLXNB2

Promoter

Availability

Academic Institutions and Nonprofits only

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TALED_Right-ND1-1397N-UGI

Plasmid

#183899

Purpose

Expresses TALED_Right-ND1-1397N-UGI in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

DDCBE for ND1 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Availability

Academic Institutions and Nonprofits only

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