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We narrowed to 6,501 results for: nls

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Showing: 181 - 200 of 6501 results

  1. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid
    #46911
    Purpose
    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain
    Depositor
    Stanley Qi, Jonathan Weissman
    Publication
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Insert
    dCas9-BFP-KRAB fusion
    Use
    CRISPR and Lentiviral
    Tags
    2xNLS, BFP, HA, and KRAB domain
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120 Articles
    46911-plasmid-map-sequence-id-449615

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  6. pEF1a-hMLH1NTD-NLS

    Plasmid
    #174826
    Purpose
    Mammalian expression of human MLH1NTD-NLS (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human MLH1_NTD-NLS (codon optimized)
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    truncation of residues 1-335
    Promoter
    EF1a
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174826-plasmid-map-sequence-id-343633

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  11. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid
    #52421
    Purpose
    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry
    Depositor
    Bertrand Collet
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion NLS-EGFP-P2A-Cherry-CAAX
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    52421-plasmid-map-sequence-id-81252

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  15. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    107942-plasmid-map-sequence-id-259760

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  17. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid
    #64219
    Purpose
    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA targeting ROSA26-1
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-BFP-P2A-E1B
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    64219-plasmid-map-sequence-id-107960

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  18. pCMV-dCas13-M3nls

    Plasmid
    #155366
    Purpose
    Targeted m6A RNA methylation in mammalian cells
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    155366-plasmid-map-sequence-id-305808

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  20. pEGFP-C1 Lifeact-EGFP-2XNLS

    Plasmid
    #58467
    Purpose
    Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Lifeact
    2X NLS with 2X stop codons
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58467-plasmid-map-sequence-id-516847

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Showing: 181 - 200 of 6501 results