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pattB-synaptobrevin-11-QFMD262-475-hsp70

Plasmid

#46120

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFMD262-475

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-8-QFAD184-214-hsp70

Plasmid

#46117

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFAD184-214

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-3-QFBD-G4MDAD-hsp70

Plasmid

#46111

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFBD-G4MDAD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-1-QFBDAD-G4MD-hsp70

Plasmid

#46109

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFBDAD-G4MD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pET28b(+)_S. cerevisiae Hbs1(162-611)

Plasmid

#39337

Depositor

SeWon Suh

Insert

Elongation factor 1 alpha-like protein

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
Gal1/10 Ura S. cerevisiae expression vector (12URA-U)

Plasmid

#48306

Depositor

Scott Gradia

Type

Empty backbone

Use

Na

Tags

Expression

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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Gal1/10 Trp S. cerevisiae expression vector (12TRP-U)

Plasmid

#48303

Depositor

Scott Gradia

Type

Empty backbone

Use

Na

Tags

Expression

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Expression

Mutation

H134R

Promoter

CAG

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pAdTrack-CMV FLAG Rev-Erb alpha

Plasmid

#22745

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

Rev Erb alpha (NR1D1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-REV1-p2A-GFP-PuroR

Plasmid

#153001

Purpose

Overexpression of human REV1

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

REV1 (REV1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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rAAV-CAG::FLEX-rev:: hM4D-2a-GFP

Plasmid

#52536

Purpose

Co-expresses hM4D and a green fluorescent label from a Cre-dependent virus

Depositor

Scott Sternson

Article

Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:

Has Service

AAV1

Insert

hM4D-2a-GFP (CHRM4 Synthetic, Human)

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

CAG

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA on ChR2

Expression

Mutation

Promoter

CBA

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

Plasmid

#101351

Purpose

HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Gal1/10 Ade S. cerevisiae expression vector (12ADE-U)

Plasmid

#48300

Depositor

Scott Gradia

Type

Empty backbone

Use

Na

Tags

Expression

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

Plasmid

#32483

Purpose

Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

ChR2HA-2a-PSAML141F,Y115F:GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

ChR2 2A

Expression

Mutation

Promoter

CAG

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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AAV Cag Flex H2B Gcamp6f reverse

Plasmid

#74155

Purpose

Calcium Sensor

Depositor

Loren Looger

Insert

Gcamp6f

Use

AAV

Tags

H2B

Expression

Mutation

Promoter

CAG

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-miR-124-sponge_rev(neg. CTRL)

Plasmid

#117326

Purpose

Dual luciferase assay negative control for miR-124 binding

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

reverse miR-124 sponge

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1-N100 S449A/T450A/S454A

Plasmid

#19849

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1-N100 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

deleted amino acids 1-100 and changed Serine 449,…

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
AAV Cag Flex H2B Gcamp6s reverse

Plasmid

#74157

Purpose

Calcium Sensor

Depositor

Loren Looger

Insert

Gcamp6s

Use

AAV

Tags

H2B

Expression

Mutation

Promoter

SpeI

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-15-LexABD-G4MD-QFAD-hsp70

Plasmid

#46124

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexABD-G4MD-QFAD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge