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We narrowed to 215 results for: stem loop

Showing: 181 - 200 of 215 results

  1. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  2. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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  3. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  14. Lenti-SAM-Puro

    Plasmid
    #198713
    Purpose
    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.
    Depositor
    Tyler Jacks
    Article
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198713-plasmid-map-sequence-id-402626

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  15. Lenti-SAM-Cre

    Plasmid
    #198714
    Purpose
    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells
    Depositor
    Tyler Jacks
    Article
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pgk and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198714-plasmid-map-sequence-id-402628

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  16. pXYB089

    Plasmid
    #239628
    Purpose
    Plant RNA Vision constructs with no gRNAs together with RFP input
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01
    stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239628-plasmid-map-sequence-id-477065

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  17. pXYB082

    Plasmid
    #239578
    Purpose
    Plant RNA Vision constructs with 41 bp gRNAs together with RFP input
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01-gRNA01
    gRNA02-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239578-plasmid-map-sequence-id-477039

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  18. pXYB083

    Plasmid
    #239579
    Purpose
    Plant RNA Vision constructs with 82 bp gRNAs together with RFP input
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01-gRNA03
    gRNA04-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    239579-plasmid-map-sequence-id-476977

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  19. pXYB156

    Plasmid
    #239632
    Purpose
    Plant RNA Vision constructs with with 82 bp gRNAs targeting Tobacco rattle virus (TRV)
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01-gRNA15
    gRNA16-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239632-plasmid-map-sequence-id-477064

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  20. pXYB085

    Plasmid
    #239637
    Purpose
    Plant RNA Vision constructs with 164 bp gRNAs together with RFP input
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01-gRNA09
    gRNA10-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239637-plasmid-map-sequence-id-477062

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Showing: 181 - 200 of 215 results