We narrowed to 223 results for: stem loop

1. pCMV-2NLS-Cas9-6XMS2

   Plasmid

   #166033

   Purpose

   Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. sgRNA(MS2) cloning backbone

   Plasmid

   #61424

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiSAMv2

   Plasmid

   #75112

   Purpose

   lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lenti sgRNA(MS2)_puro backbone

   Plasmid

   #73795

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiX1-[TRE3G-TS.FF4x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235316

   Purpose

   Lentiviral expression of mRuby2 with 5' microRNA target site only (closed-loop)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLentiX1-[TRE3G-TS.FF6x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235323

   Purpose

   Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 3

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiX1-[TRE3G-TS.FF6x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235315

    Purpose

    Lentiviral expression of mRuby2 with 5' microRNA target site only (open-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiX1-[TRE3G-TS.FF6x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235321

    Purpose

    Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentiX1-[TRE3G-mRuby2-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235313

    Purpose

    Lentiviral expression of mRuby2 with 3' microRNA target site only (open-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235320

    Purpose

    Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235319

    Purpose

    Lentiviral expression of ComMAND open-loop circuit regulating mRuby2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235314

    Purpose

    Lentiviral expression of mRuby2 with 3' microRNA target site only (closed-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiX1-[TRE3G-TS.FF4x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235322

    Purpose

    Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiX1-[TRE3G-TS.FF4x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235324

    Purpose

    Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 3

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-SAM-Puro

    Plasmid

    #198713

    Purpose

    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a and U6

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-SAM-Cre

    Plasmid

    #198714

    Purpose

    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    Pgk and U6

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXYB089

    Plasmid

    #239628

    Purpose

    Plant RNA Vision constructs with no gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01
    stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge