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Addgene

We narrowed to 5,034 results for: plasmid dna

Showing: 3961 - 4000 of 5034 results
  1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-7

    Plasmid
    #129059
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting mouse TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA7 (mouseTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-8

    Plasmid
    #129060
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting mouse TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA8 (mouseTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

    Plasmid
    #129047
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA7 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA7 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

    Plasmid
    #129049
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA9 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid
    #129050
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA10 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid
    #129051
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA11 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

    Plasmid
    #129052
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA12 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA12 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

    Plasmid
    #129040
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA12 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid
    #129042
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA2targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA2 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid
    #129043
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA3 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA3 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129044
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA4 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

    Plasmid
    #129045
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA5 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid
    #129046
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA6 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

    Plasmid
    #129033
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA5 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA5 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid
    #129034
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA6 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

    Plasmid
    #129035
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA7 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

    Plasmid
    #129037
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA9 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid
    #129038
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA10 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid
    #129039
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA11 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid
    #129030
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA2 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid
    #129031
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA3 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129032
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA4 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  23. PVALB mAb _BCN14.1.1C9_HC_Mouse IgG2a

    Plasmid
    #182888
    Purpose
    Mammalian expression of PVALB mouse mAb HC gene
    Depositor
    Insert
    anti-PVALB (human) heavy chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  24. PVALB mA_BCN14.1.1C9_LC_Mouse Ig Kappa

    Plasmid
    #182889
    Purpose
    Mammalian expression of PVALB mouse mAb LC gene
    Depositor
    Insert
    anti-PVALB (human) light chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  25. PVALB mAb_BCN14.1.1C9_LC_Rat Ig Kappa

    Plasmid
    #182891
    Purpose
    Mammalian expression of PVALB rat mAb LC gene
    Depositor
    Insert
    anti-PVALB (human) light chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  26. PVALB mAb_BCN14.1.1A2_LC_Mouse Ig Kappa

    Plasmid
    #182893
    Purpose
    Mammalian expression of PVALB mouse mAb LC gene
    Depositor
    Insert
    anti-PVALB (human) light chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  27. KG#121

    Plasmid
    #110879
    Purpose
    Expresses the C. elegans unc-31 cDNA pan-neuronally
    Depositor
    Inserts
    rab-3 promoter
    unc-31 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Missing first 408bp
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  28. KG#371

    Plasmid
    #110882
    Purpose
    Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons
    Depositor
    Inserts
    unc-129 promoter
    CTNS-1a-mCherry cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Use
    Tags
    intron-mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  29. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
  30. pHR_SFFV*/Puro2a-GFP

    Plasmid
    #198222
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Codon-optimized puromycin resistance gene, follow…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  31. pHW1387

    Plasmid
    #229880
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Depositor
    Insert
    Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Use
    Tags
    GFP-C1
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  32. pHW1392

    Plasmid
    #229878
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses- Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Depositor
    Insert
    Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Use
    Tags
    GFP-C1
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  33. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

    Plasmid
    #114078
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
    Use
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 3961 - 4000 of 5034 results