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pBK-CMV Pig10

Plasmid

#16504

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig10 (ENC1 Human)

Expression

Mammalian

Available Since

May 12, 2010

Availability

Academic Institutions and Nonprofits only
pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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H6hSDHA

Plasmid

#113045

Purpose

Hetero expression of His-tagged human SDHA

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

H6hSDHA (SDHA Human)

Tags

His6 (MGSSHHHHHHSQDP-)

Expression

Bacterial

Mutation

Insert begins at amino acid 43

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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p32Fn

Plasmid

#158153

Purpose

Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Mutation

Rice codon optimized

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-MAHS

Plasmid

#90034

Purpose

To express GFP-tagged MAHS in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

Plasmid

#79373

Purpose

Recruits VPR to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-VPR

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

Plasmid

#79372

Purpose

Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-p65-hsf1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRL c-Myc 3'UTR

Plasmid

#14806

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

c-Myc 3'UTR (Myc Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pCZGY847

Plasmid

#135093

Purpose

Transcriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegans

Depositor

Yishi Jin

Article

Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

Insert

Pacr-2

Tags

mcherry and unc-10 3'UTR

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-TOP3B

Plasmid

#225470

Purpose

Express mEGFP-fusion protein of TOP3B

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

TOP3B (TOP3B Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCLC3sHA

Plasmid

#52424

Purpose

mammalian expression of HA tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Wang et al Gastroenterology. 2004 Apr;126(4):1157-6

Insert

CLC3 (Clcn3 Rat)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSG5-FER

Plasmid

#30191

Depositor

Nora Heisterkamp

Article

Hao et al Mol Cell Biol. 1989 Apr . 9(4):1587-93.

Insert

Fer tyrosine kinase (FER Human)

Expression

Mammalian

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
Jnk3a1

Plasmid

#29577

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Jnk3a1 (MAPK10 Human)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Contains residues 40-402

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only
RFP-KIF3A-HL

Plasmid

#51397

Purpose

expression of RFP-tagged amino acid 369-701 (headless) of KIF3A in mammalian cells

Depositor

Trina Schroer

Article

Brown et al Traffic. 2005 Dec;6(12):1114-24.

Insert

KIF3A (Kif3a Mouse)

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pC002 - LshC2C2 locus into pACYC184

Plasmid

#79151

Purpose

Endogenous LshC2c2 locus cloned into pACYC184

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Expression

Bacterial

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140168

Purpose

Expresses a regulatable secretory cargo for Tetrahymena thermophila

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Other

Promoter

MTT1

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE- TRE-DEST-NBioTAP

Plasmid

#53568

Purpose

Lentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pISH-Barx1

Plasmid

#84306

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Barx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Barx1 (Barx1 Mouse)

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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CAGhP14

Plasmid

#84362

Purpose

express FLAG-HA tagged human PRDM14

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CAG

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only