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We narrowed to 4,326 results for: CIT

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Showing: 1981 - 2000 of 4326 results

  4. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Available Since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

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  5. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Article
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    ∆exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available Since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

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  6. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

    Plasmid
    #124299
    Purpose
    Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.
    Insert
    UBE2G1 (UBE2G1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    CMV
    Available Since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124299-plasmid-map-sequence-id-244604

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  12. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331070

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  13. pTE3327

    Plasmid
    #107535
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RVR mutant)
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K548V, N552R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107535-plasmid-map-sequence-id-207331

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  14. pTE3328

    Plasmid
    #107539
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RR mutant)
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K607R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107539-plasmid-map-sequence-id-207236

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Showing: 1981 - 2000 of 4326 results