We narrowed to 157,100 results for: Des

1. pCSDest2-NmeCas9-NLS-3XHA-NLS

   Plasmid

   #87448

   Purpose

   Mammalian expression of wild type Nme Cas9

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Nme Cas9
   

   Use

   CRISPR

   Tags

   3XHA, SV40 NLS, and cMyc-like NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6268 pDEST-59-His-Trx-HPV16E6

   Plasmid

   #24126

   Depositor

   Peter Howley

   Insert

   HPV16 E6 (E6, E6 HPV)
   

   Tags

   His and Trx

   Expression

   Bacterial and Mammalian

   Mutation

   none

   Available Since

   March 9, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

   Plasmid

   #187154

   Purpose

   mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDN0606 (pDEST-DHFR F[3] N-term,HygR)

   Plasmid

   #210488

   Purpose

   pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKB12 (pDEST-DHFR F[3] C-term, HygR)

   Plasmid

   #210486

   Purpose

   pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

   Plasmid

   #136513

   Purpose

   Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

   Insert

   codon-optimized AcrIIA5
   

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-Spe4-Kozak-Snaptag-RfA

   Plasmid

   #186385

   Purpose

   Destination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAdCMV/V5-DEST-S727A-STAT3-3xFlag

   Plasmid

   #99262

   Purpose

   Adenoviral vector encoding Flag-tagged murine STAT3 (S727A)

   Depositor

   Alejandro Adam

   Article

   Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

   Insert

   Signal transducer and activator of transcription 3 - S727A (Stat3 Mouse)
   

   Use

   Adenoviral

   Tags

   3x-Flag

   Mutation

   S727A

   Promoter

   CMV

   Available Since

   Jan. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiPGK Puro DEST JNKKTR AE Clover

   Plasmid

   #90240

   Purpose

   Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (AE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT[Hygro]_GBP-3xFlag-DEST

    Plasmid

    #157813

    Purpose

    A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection

    Depositor

    Zoltan Lipinszki

    Article

    Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:

    Insert

    GFP-binding protein
    

    Use

    For drosophila cells

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    metallothionein

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC-DEST-cjBLIMP1-Venus-HygTK

    Plasmid

    #141028

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    mVenus
    

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAdCMV/V5-DEST-S727D-STAT3-3xFlag

    Plasmid

    #99263

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3 (S727D)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - S727D (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Mutation

    S727D

    Promoter

    CMV

    Available Since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-CMV 3xFLAG-LC3B2-GFP

    Plasmid

    #123109

    Purpose

    Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

    Plasmid

    #51255

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Ala

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pDEST-Spe4-RfA-emiRFP703-stop

    Plasmid

    #186386

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid

    #51257

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248,789,805,817,848,9…

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

    Plasmid

    #85750

    Purpose

    Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Type II-C anti-CRISPR AcrIIC3Nme
    mTagBFP2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid

    #51256

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Glu

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. IZ401: pMVP/Lenti/Neo-DEST

    Plasmid

    #121850

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

    Plasmid

    #85749

    Purpose

    Expresses anti-CRISPR protein from Type I-E and mTagBFP2

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Type I-E anti-CRISPR
    mTagBFP2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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