We narrowed to 165,567 results for: addgene

1. pX459_gRNA-ROSA26_hspCas9

   Plasmid

   #193310

   Purpose

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-GfaABC1D-GRAB_Ado1.0

   Plasmid

   #153288

   Purpose

   Express the adenosine sensor GRAB_Ado1.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

   Insert

   Adenosine sensor GRAB_Ado1.0
   

   Use

   AAV

   Promoter

   GfaABC1D

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIG-Aalpha WT

   Plasmid

   #10884

   Depositor

   William Hahn

   Article

   Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

   Insert

   PP2A Aalpha (PPP2R1A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVPT-tTR-KRAB

   Plasmid

   #11642

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. MXS_CMV::rtTA3-bGHpA

   Plasmid

   #62445

   Purpose

   MXS_chaining vector with CMV::rtTA3-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the reverse tetracycline transactivator with CMV promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVET-tTR-KRAB

   Plasmid

   #11644

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hEF-1alpha, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. 7MEQ

   Plasmid

   #176412

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
   

   Use

   Baculovirus expresssion

   Tags

   8 x His Tag and Honeybee melittin signal

   Mutation

   etmprss2_S251D/R252D/Q253D/S254D/R255K

   Promoter

   Polyhedrin

   Available Since

   Oct. 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pNup133-Ypet

   Plasmid

   #98283

   Purpose

   mammalian expression of Nup133-Ypet

   Depositor

   Mike Heilemann

   Article

   Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

   Insert

   Nup133 (NUP133 Human)
   

   Tags

   YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE puro PPAR gamma 2

   Plasmid

   #8859

   Depositor

   Bruce Spiegelman

   Article

   Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

   Insert

   PPAR gamma (Pparg Mouse)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNH-NanoLuc

    Plasmid

    #173075

    Purpose

    Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p206_LTJ_sgRNACD45.2_R3

    Plasmid

    #82674

    Purpose

    sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. LES2A-CRE

    Plasmid

    #167933

    Purpose

    Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    mScarlet-P2A-CRE
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEVY-L

    Plasmid

    #51226

    Purpose

    bi-directional expression vector for yeast, constitutively active, LEU2 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GPD/ADH1

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-dCasRx-ccdB

    Plasmid

    #221003

    Purpose

    Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. iMbody_pSANG10-3F

    Plasmid

    #184496

    Purpose

    Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

    Depositor

    Marcel Dinger

    Article

    Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

    Insert

    iMbody
    

    Tags

    3xFLAG, 6xHis, and pelB leader

    Expression

    Bacterial

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe NEO mRFP1

    Plasmid

    #98397

    Purpose

    Retroviral vector for constitutive mRFP1 expression

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    mRFP1
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMMP-MCS-IRES-mRFP

    Plasmid

    #36972

    Depositor

    Bill Sugden

    Article

    Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. HaloKu70 sgRNA

    Plasmid

    #207583

    Purpose

    sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    GAGCAGTAGCCAACATGTCA
    

    Expression

    Mammalian

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. M4754 KanMX::HIS3 Disruptor Converter

    Plasmid

    #51684

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to HIS3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    HIS3 (HIS3 Budding Yeast)
    

    Use

    Yeast marker swap

    Expression

    Yeast

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. EdTC

    Plasmid

    #24310

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-mCherry (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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