We narrowed to 11,710 results for: pcDNA

1. pCDNA5/FRT-ChemoG5

   Plasmid

   #193807

   Purpose

   CMV overexpression of ChemoG5 in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG5
   

   Use

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   EGFP:A206K, T225R; HT7: E143R, E147R, L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-FLAG-mTET2 (CD)

   Plasmid

   #89736

   Purpose

   Expresses the CD-domain truncation to mouse TET2 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   CD (cysteine-rich, dioxygenase domain) truncation…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1his p66shc (corrected)

   Plasmid

   #32574

   Depositor

   Toren Finkel

   Article

   Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

   Insert

   p66shc (SHC1 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-DN-hCUL4B-FLAG

   Plasmid

   #15822

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005 . 399():287-309.

   Insert

   Cullin4B (CUL4B Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N594

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-MCS-EGFP_AB

   Plasmid

   #148304

   Purpose

   Mammalian Expression vector

   Depositor

   Elisa Izaurralde

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT-TO-Nsp13_GC3opt

   Plasmid

   #157714

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pcDNA3-DN-hCUL5-FLAG

   Plasmid

   #15823

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005 . 399():287-309.

   Insert

   Cullin5 (CUL5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N441

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3 IRF7(5’UTR)-FF

   Plasmid

   #85490

   Purpose

   Firefly luciferase under the control of IRF7 5'UTR

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   5'UTR of IRF7 (Irf7 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-mCherry-CyrCR1

   Plasmid

   #128768

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-HA-mRFP-pcDNA3.1-

    Plasmid

    #52510

    Purpose

    expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    mRFP
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    engineered variant of tetrameric red fluorescent …

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.2/v5-DEST hGlut1

    Plasmid

    #18085

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    Glut1 (SLC2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA4/TO/GFP-GIGYF2

    Plasmid

    #141189

    Purpose

    Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    GIGYF2 (GIGYF2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-Lyso-InPAkt

    Plasmid

    #184053

    Purpose

    Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Lyso-InPAkt
    

    Use

    Tags

    Lysosome-associated membrane protein 1 (LAMP1).

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)-ExRai-AKAR2(T/A)

    Plasmid

    #161754

    Purpose

    Negative-control mutant for ExRai-AKAR2 biosensor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2(T/A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO V5 HSPA8

    Plasmid

    #19514

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 8 (HSPA8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-pmExRai-AKAR2

    Plasmid

    #161755

    Purpose

    Plasma membrane-targeted ExRai-AKAR2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    Lyn-ExRai-AKAR2
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3/hArf5(WT)-mCherry

    Plasmid

    #79421

    Purpose

    Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-1xHA CARM1 WT

    Plasmid

    #81118

    Purpose

    mammalian expression of CARM1 WT

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    CARM1 (Carm1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-ROS1-V5/HIS

    Plasmid

    #205609

    Purpose

    Expression of human ROS1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROS1 receptor tyrosine kinase (ROS1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin2 (CUL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge