178,101 results
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Plasmid#97410PurposeAmplified expression of GCaMP6f in combination with TET driverDepositorInsertGCaMP6f
UseAAVExpressionMammalianPromoterTRE3Available sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_Thy1StTA
Plasmid#97411PurposeTET driver for amplified expression in cortical neuronsDepositorInserttTA
UseAAVExpressionMammalianPromotermouse thy1PSsAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.Flex.GCaMP6s.WPRE.SV40
Plasmid#100845PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-Cas9-162A
Plasmid#84918Purposefor in vitro transcription of Cas9 with polyA tail encodedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
TagsFlag, NLS, and polyA tailExpressionMammalianPromoterCAGAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G9A[SET]-dCas9
Plasmid#100089PurposeCatalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG9A (EHMT2 Human)
UseCRISPRTags3XFLag-NLS-G9A[SET]-dCas9-NLSExpressionMammalianMutationG9A[SET] includes aa 829-1209PromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT EGFP-miniAID
Plasmid#101714PurposeFor amplification of a EGFP-miniAID for N-terminal taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-miniAID
TagsEGFP-miniAIDExpressionMammalianAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGF1-4eCOL2A1
Plasmid#97210PurposeLentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoterDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseLentiviral and LuciferaseExpressionMammalianPromoterCOL2A1 regulatory elementsAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-ihSyn1-DIO-tTA
Plasmid#99121PurposeAn AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTADepositorHas serviceAAV1InsertDIO-tTA
UseAAVExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YIp204-PADH1-atTIR1-9myc
Plasmid#99532PurposeS. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5
Plasmid#97044PurposeExpresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertLigand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
UseLentiviralExpressionMammalianPromoterTet onAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only