174,630 results
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Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
Tags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-EGFP-caax
Plasmid#190155PurposeExpresses membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorHas ServiceAAV1 and AAV8InsertEGFP-caax
UseAAVExpressionMammalianPromoterMBPAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)
Plasmid#185911PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-GINKO2
Plasmid#177116PurposeBacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2DepositorInsertGINKO2
Tags6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…ExpressionBacterialAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBE14
Plasmid#174653PurposeIn vivo Cre-lox circularization in E. coliDepositorInsertCre
UseSynthetic BiologyAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorInsertcytoEKAR4
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLIN2-V5-APEX2
Plasmid#170573PurposeA C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2DepositorAvailable SinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -