We narrowed to 4,186 results for: GCA

1. EcPhnD90.L1∆∆AD.L297RL301R.pRSET

   Plasmid

   #33376

   Purpose

   a single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFP

   Depositor

   Loren Looger

   Article

   Alicea et al J Mol Biol. 2011 Oct 12.

   Insert

   phnD based Phosphonate Biosensor (phnD E. coli)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted in place of…

   Promoter

   T7

   Available since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458_SOX5_1

   Plasmid

   #86357

   Purpose

   Encodes gRNA for 3' target of human SOX5

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against SOX5 (SOX5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-sgRNA_Dgcr8_2

   Plasmid

   #73526

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

4. shCDK6(2)

   Plasmid

   #73553

   Purpose

   shRNA(2) against CDK6

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK6
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pRCA360 - pBA904 Puro-T2A-GFP

   Plasmid

   #238164

   Purpose

   Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

   Insert

   Non-targeting sgRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-IARS2-tb

   Plasmid

   #232340

   Purpose

   Expresses human IARS2 with synonymous mutations (to prevent recognition by siRNA) in mammalian cells

   Depositor

   Quanfu Ma

   Insert

   IARS2 (IARS2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5 synonymous mutations in the siRNA (ACUUGCAGUCAU…

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. esgRNA_NbmiR164e/h

   Plasmid

   #231157

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting NbmiR164e/h

   Depositor

   Daniel Voytas

   Article

   Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

   Insert

   mobile gRNA targeting NbmiR164e/h
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. esgRNA_SlMIR164b

   Plasmid

   #231153

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting SlMIR164b

   Depositor

   Daniel Voytas

   Article

   Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

   Insert

   mobile gRNA targeting SlMIR164b
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYAMTr2GCsgSeLEU2

   Plasmid

   #224870

   Purpose

   Disruption of S. eubayanus type LEU2 genes

   Depositor

   Katsunori Suzuki

   Article

   Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

   Insert

   pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA_SRR124.5_tagBFP

    Plasmid

    #163753

    Purpose

    gRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    gRNA_SRR124.5
    

    Use

    CRISPR

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid

    #221844

    Purpose

    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GACG and U6.3 chick

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LV-sgRNA-mCherry-Puro sgPIDD

    Plasmid

    #211528

    Purpose

    Deletes PIDD

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgPIDD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210744

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 4
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid

    #208979

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210742

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 2
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKE011

    Plasmid

    #207912

    Purpose

    Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS018

    Plasmid

    #207911

    Purpose

    Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459-dErbB1

    Plasmid

    #197354

    Purpose

    A knock-out vector for dog ErbB1.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMpGE010-Target1 (Mpphot)

    Plasmid

    #186728

    Purpose

    Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

    Depositor

    Yutaka Kodama

    Article

    Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

    Insert

    Mphot
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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