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Plasmid#215008PurposeExpress a Lysophagy reporter in mammalian cells (untested)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRS-Leader-SARS-CoV-2-EGFP
Plasmid#171585PurposeExpression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporterDepositorInsertTRS-Leader-SARS-CoV-2-d2eGFP
UseLentiviralTags2PEST AND NLSExpressionMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L
Plasmid#122991PurposeExpresses Flag-tagged hRPGRIP1L in mammalian cellsDepositorInsertRPGRIP1L (RPGRIP1L Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1 alphaAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-CDK6-mutPAM-PGK-puro
Plasmid#187638Purposesg resistant CDK6 ORFDepositorInsertCDK6 (CDK6 Human)
UseLentiviralTagsExpressionMammalianMutationPAM site mutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-V12-puro
Plasmid#156168PurposeLentiviral vector for expression of flag-RAP1B-V12, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV-MRLC1-mRuby2-IRES-PuroR
Plasmid#103031PurposeLentiviral expression of mRuby2-tagged MRLC with puromycin selectionDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT 5N5A (Puro)
Plasmid#154293PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationN322A, N356A, N390A, N424A, N458A mutations; HCF-…PromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) MXRA7
Plasmid#107500PurposeExpresses MXRA7, puromycin resistantDepositorInsertMXRA7 (codon optimized) (MXRA7 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Triple ORF LoxP ifgMosaic Donor (LH208)
Plasmid#99625PurposeTo clone the 3 ORFs for Cre dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/Lox, Mouse Targeting, and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-STOP-IRES-Puro
Plasmid#120558PurposeExpresses control HA-birA* in mammalian cells & for virus productionDepositorInsertbirA
UseLentiviralTagsHAExpressionMammalianMutationR118GPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF2-V2-FLAG-IRES-GFP
Plasmid#69049Purposelentiviral expression of IKZF2 variant 2 with FLAG tagDepositorInsertIKZF2 (IKZF2 Human)
UseLentiviralTagsFLAG and IRES-GFPExpressionMammalianMutationsplice variant 2PromoterCAGAvailable sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-Myc-CRBN-pGK-HYG
Plasmid#107376PurposeLentiviral expression of myc-CRBNDepositorInsertcereblon (CRBN Human)
UseLentiviralTagsMycExpressionMammalianMutationPromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast
Plasmid#71218PurposedCas9 fused to the human DNMT3A catalytically inactive domainDepositorInsertdSpCas9-DNMT3A(E752A)
UseLentiviralTagsSPdCas9-DNMT3A(E752A)ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur
Plasmid#69051Purposelentiviral expression of IKZF1 with HA tagDepositorInsertIKZF1 (IKZF1 Human)
UseLentiviralTagsHAExpressionMammalianMutationsplice variant 2PromoterUBCAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP
Plasmid#204821PurposeLentiviral vector for human SCGB3A2 promoter-driven expression of EGFPDepositorInsertAn upstream region including human SCGB3A2 promoter
UseLentiviralTagsExpressionMammalianMutationPromoterSCGB3A2 promoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iD20 LoxStopLox-Zim3-dCas9-mApple-hygro
Plasmid#204720PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) VOPP1
Plasmid#107507Purposeexpressing VOPP1, puromycin resistantDepositorInsertVOPP1 (VOPP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-P2A-GFP-PGK-Puro
Plasmid#110868PurposeLentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HNRNPR
Plasmid#200730PurposeLentiviral expression of Flag-Tagged human HNRNPRDepositorInsertHNRNPR (HNRNPR Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) FAM149A
Plasmid#107510PurposeExpressing FAM149a, puromycin resistantDepositorInsertFAM149A (FAM149A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only