We narrowed to 4,947 results for: mcs

1. pAAV_AR-RE_MLPmin_BC1014-luc2

   Plasmid

   #227108

   Purpose

   Androgen receptor response element for luciferase and barcode assays; barcode BC1014

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. G1333 DddAtox-N–SaKKH-Cas9(D10A)

   Plasmid

   #157837

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-3myc-6His-EZH2 21D

   Plasmid

   #42664

   Depositor

   Mien-Chie Hung

   Article

   Cha et al Science. 2005 Oct 14;310(5746):306-10.

   Insert

   EZH2 (EZH2 Human)
   

   Tags

   3Myc and 6His

   Expression

   Mammalian

   Mutation

   S21D

   Available Since

   July 17, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET21-pelB-LaM-3

   Plasmid

   #172769

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-3
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRMC2-tat:msfGFP

   Plasmid

   #194915

   Purpose

   Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Expression

   Bacterial

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_ER-RE_MLPmin_BC1063-luc2

   Plasmid

   #227109

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-3myc-6His-EZH2 21A

   Plasmid

   #42663

   Depositor

   Mien-Chie Hung

   Article

   Cha et al Science. 2005 Oct 14;310(5746):306-10.

   Insert

   EZH2 (EZH2 Human)
   

   Tags

   3Myc and 6His

   Expression

   Mammalian

   Mutation

   S21A

   Available Since

   July 17, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-Zeo_BACE1

   Plasmid

   #227105

   Purpose

   BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   BACE1 (BACE1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mAMCase_CatD_6xHis_pTwistCMV

   Plasmid

   #200228

   Purpose

   Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

   Depositor

   James S Fraser

   Article

   Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

   Insert

   Acidic Mammalian Chitinase (Chia1 Mouse)
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S354F

   Promoter

   CMV

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. JDW 947 (pME-mmTcf4_DN_P2A_mCherry)

    Plasmid

    #232122

    Purpose

    A middle entry gateway clone containing myc-tagged murine dominant negative Tcf4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    Tcf4ΔN (Tcf4 Mouse)
    

    Use

    Gateway cloning

    Tags

    myc

    Mutation

    lacks aa 1-56

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV7.1_3xFLAG-STK4

    Plasmid

    #172989

    Purpose

    constitutive expression of FLAG-tagged STK4

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-STK4 (STK4 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

    Plasmid

    #193469

    Purpose

    Cp36 Donor with promoter for mcherry and puro expression

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV7.1_3xFLAG-WWC3

    Plasmid

    #172996

    Purpose

    constitutive expression of FLAG-tagged WWC3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-WWC3 (WWC3 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pESC-URA::CYP71AV1/CPR

    Plasmid

    #20118

    Depositor

    Jay Keasling

    Article

    Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

    Insert

    amorphadiene oxidase and p450 reductase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 2, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pET21-pelB-LaM-2

    Plasmid

    #172768

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ND4 right TALE–G1333 DddAtox C

    Plasmid

    #158095

    Purpose

    expresses the right-side ND4 TALE–DddAtox half in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-C–1xUGI
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. ND4 left TALE–G1333 DddAtox N

    Plasmid

    #158093

    Purpose

    expresses the left-side ND4 TALE–DddAtox half in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-N–1xUGI
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBK_Gal4-DBD-GR(NR3C1)

    Plasmid

    #227100

    Purpose

    GR receptor overexpression for luciferase and barcode assays

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    GR (NR3C1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge