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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-FLEx(FRT)-TC

Plasmid

#67827

Purpose

Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

TVA-mCherry fusion

Use

AAV

Expression

Mammalian

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP Tensin1

Plasmid

#105297

Purpose

To study localization and function of Tensin1

Depositor

David Critchley, Kenneth Yamada

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin1 (TNS1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).cpSFGFP.mRuby3

Plasmid

#244914

Purpose

cpSFGFP control with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mRuby3

Use

AAV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cre-HiBiT

Plasmid

#198318

Purpose

Loads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

Plasmid

#171437

Purpose

Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

c-Fos-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

Plasmid

#171427

Purpose

Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-FRB

Expression

Mammalian

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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NbV5-HALOTAG

Plasmid

#201482

Purpose

Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HALOTAG

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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TtTMPV-Neo

Plasmid

#27993

Purpose

Retroviral transfer plasmid for Tet-regulated shRNA expression with a TREtight promoter. Contains TurboRFP and Venus fluorescent reporters.

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TtTMPV-Neo (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pHR iSH-YFP-PIF

Plasmid

#50841

Purpose

Optogenetic PI3K activation system; cytoplasmic component

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Inserts

p85 iSH2
PIF6 1-100

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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UAS:Zebrabow-V

Plasmid

#118218

Purpose

For broad or variegated gal4 inducible expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-Vinexin-C-14

Plasmid

#54305

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Vinexin (SORBS3 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMSCV LDTS-LIRmut

Plasmid

#189001

Purpose

lipophagy-inducing adaptor protein, negative control

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-LIRmut

Use

Retroviral

Tags

HA

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-FLEx(FRT)-G

Plasmid

#67828

Purpose

Flp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

rabies glycoprotein

Use

AAV

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

Plasmid

#171438

Purpose

Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-c-Jun

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2low-tdTomato

Plasmid

#104067

Purpose

Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2low-tdTomato

Expression

Mammalian

Mutation

CRY2 aa 1-491 truncation, R489E A491D

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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GAG-Pan

Plasmid

#99640

Purpose

EGFP-mCherry Met71GAG reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Tags

EGFP

Expression

Mammalian

Mutation

Met71 mutated to GAG (Glu)

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209726

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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GAA-Pan

Plasmid

#99639

Purpose

EGFP-mCherry Met71GAA reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Tags

EGFP

Expression

Mammalian

Mutation

Met71 mutated to GAA (Glu)

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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