165,775 results

1. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

   Plasmid

   #126217

   Purpose

   Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   Gephyrin.FingR-eGFP-CCR5TC
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLIB-GST-3C-CDK1

   Plasmid

   #177011

   Purpose

   Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CDK1 (CDK1 Human)
   

   Tags

   GST-3C

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-PKCA D463N

   Plasmid

   #195203

   Purpose

   Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

   Depositor

   David Bryant, Peter Parker

   Article

   Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

   Insert

   PRKCA (PRKCA Human)
   

   Expression

   Mammalian

   Mutation

   D463N

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMP71-HLA-A0201-His

   Plasmid

   #108214

   Purpose

   HLA-A0201 his tagged

   Depositor

   Sébastien Walchli

   Article

   Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

   Insert

   major histocompatibility complex, class I, B (HLA-A Human)
   

   Use

   Retroviral

   Tags

   his

   Expression

   Mammalian

   Available Since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-LIC

   Plasmid

   #26094

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Lenti‐Cas9‐2A‐Blast

   Plasmid

   #73310

   Purpose

   Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

   Plasmid

   #118617

   Purpose

   Lentiviral expression of mTurq2 tagged PCNA

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   TK-NLS-mTurq2-PCNA (PCNA Human)
   

   Use

   Lentiviral

   Tags

   mTurq2

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-ERalpha

   Plasmid

   #215093

   Purpose

   Expresses HA-ERalpha in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   ESR1 (ESR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6P-1_WT RAD23B

   Plasmid

   #201457

   Purpose

   Expresses human RAD23B with an N-terminal GST tag in E. coli

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   tac promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. gTBEv4

    Plasmid

    #220620

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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