19,627 results

1. EGFP-p65

   Plasmid

   #111190

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

   Insert

   RELA (RELA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. HPSE-bio-His

   Plasmid

   #53407

   Purpose

   Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   HPSE (HPSE Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJRH-1179 U6-reci Gag-Cas9 v2

   Plasmid

   #201914

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Gag-Cas9 v2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP Kindlin2

   Plasmid

   #105305

   Purpose

   To study effect of Kindlin 2 on Cell/ECM adhesion

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

   Plasmid

   #31482

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (Sce Target) EF Puro
   

   Use

   Lentiviral

   Mutation

   mCherry has M9S and M16L mutations. This is what …

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYD1

   Plasmid

   #73447

   Purpose

   expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

   Depositor

   Dane Wittrup

   Article

   Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

   Type

   Empty backbone

   Tags

   6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYC-GroEL/ES-TF

   Plasmid

   #83923

   Purpose

   Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Gro EL/ES
   Trigger Factor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-ATF6

   Plasmid

   #32955

   Purpose

   For visualization of GFP-ATF6 in mammalian cells.

   Depositor

   Ron Prywes

   Article

   Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

   Insert

   ATF6 (ATF6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   M67L and M313I

   Promoter

   CMV

   Available Since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRPN265NB-enh-MNase

   Plasmid

   #166035

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

   Insert

   Glutathione-S-Transferase/enhancer/MNase
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   lac promoter / tac promoter

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAIDFA-EF1a-NmScarlet-mAID

    Plasmid

    #140615

    Purpose

    Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1 mutated F74A
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-Gal3

    Plasmid

    #85662

    Purpose

    Fluorescent reporter for lysosomal damage

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    LGALS3 (LGALS3 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid

    #103865

    Purpose

    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Contains the H133A and H1058A mutations which ina…

    Promoter

    EF1a

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEIGW Grx1-roGFP2

    Plasmid

    #64990

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid

    #20298

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1_blasti

    Plasmid

    #194067

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPRv2 hygro

    Plasmid

    #98291

    Purpose

    Variant of lentiCRISPRv2 that confers hygromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. E-cadherin-GFP

    Plasmid

    #28009

    Depositor

    Jennifer Stow

    Article

    Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

    Insert

    E-cadherin (CDH1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAMD1-4

    Plasmid

    #61565

    Purpose

    Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCA24N-ligase

    Plasmid

    #87741

    Purpose

    IPTG-inducible expression of T4 DNA ligase for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1α-Cre-2A-Puro

    Plasmid

    #207743

    Purpose

    Expression of the Cre recombinase linked to a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-2A-Puro
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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