162,160 results

1. pGWB501NL3F10H

   Plasmid

   #141286

   Purpose

   No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Type

   Empty backbone

   Use

   Luciferase; Gateway

   Tags

   FLAG (x3), HIS (x10), and NLUC

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42

   Plasmid

   #111746

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q42 (polyQ repeat)

   Promoter

   CMV and P10

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-p47PX(aa2-132)

   Plasmid

   #19007

   Depositor

   Michael Yaffe

   Article

   Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

   Insert

   p47phox (NCF1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa2-132 (PX domains)

   Promoter

   Available since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-TRE-DIO-TVA-oG-mCherry

   Plasmid

   #166602

   Purpose

   Encodes Cre-dependent TVA-oG-mCherry under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   TVA-oG-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPRia-v2

   Plasmid

   #84832

   Purpose

   CRISPRi/a V2 library parental plasmid

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Inserts

   sgRNA
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mEmerald(luminal)-canxTM

   Plasmid

   #186961

   Purpose

   Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   CANX (CANX Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Only express calnexin (482-end) with ER signal pe…

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLCAR-CD19-BBz

   Plasmid

   #135992

   Purpose

   Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSCV-FKBP-STING(pLxIS)

   Plasmid

   #221240

   Purpose

   Expression of STING CTT in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   STING CTT (Sting1 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221-Basigin_iso2

   Plasmid

   #221416

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   Basigin_iso2 (BSG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ORCO-T2A-QF-DBD

    Plasmid

    #162525

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX Sap-SH2

    Plasmid

    #46477

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Sap (SH2D1A Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 4-128

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194381

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTol2-elavl3_NES-rsCaMPARI-mRuby3

    Plasmid

    #122129

    Purpose

    Pan-neuronal expression of rsCaMPARI in zebrafish, nucleus excluded

    Depositor

    Eric Schreiter

    Article

    Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

    Insert

    rsCaMPARI
    

    Use

    Zebrafish expression

    Tags

    NES-His and mRuby3

    Expression

    Mutation

    Promoter

    elavl3

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDM1485

    Plasmid

    #207446

    Purpose

    Extrachromosomal expression of GFP-MhcA

    Depositor

    Rob Kay

    Article

    Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

    Insert

    mhcA
    

    Use

    Unspecified

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV_Npu-saBE3.9max C-terminal

    Plasmid

    #137180

    Purpose

    Expresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    Npu-saBE3.9max_C-terminal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV_Npu-saBE3.9max N-terminal

    Plasmid

    #137179

    Purpose

    Expresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    Npu-saBE3.9max_N-terminal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 D10A

    Promoter

    CMV

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMT-IMD-V5-His

    Plasmid

    #63748

    Purpose

    Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    immune deficiency (imd Fly)
    

    Use

    Tags

    V5-6XHis

    Expression

    Insect

    Mutation

    *see comment below

    Promoter

    pMT

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HSPB1

    Plasmid

    #155670

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    HSPB1 (HSPB1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX SHP-2(NC)-SH2

    Plasmid

    #46499

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-2(NC) (PTPN11 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-222

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sortase A pentamutant (eSrtA) in pET29

    Plasmid

    #75144

    Purpose

    evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

    Depositor

    David Liu

    Article

    Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

    Insert

    sortase A pentamutant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T. Deleted amino a…

    Promoter

    T7

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge