We narrowed to 12,201 results for: 112

1. pCR1306

   Plasmid

   #111128

   Purpose

   Expresses FANCE.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCE (FANCE Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2-7xTcf-NLS-CNL

   Plasmid

   #65712

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL
   

   Use

   Luciferase

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRT/FLAG/HA-DEST LIN28B

   Plasmid

   #43798

   Depositor

   Thomas Tuschl

   Article

   Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

   Insert

   LIN28B (LIN28B Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUBC_HA_PP7-mVenus

   Plasmid

   #111268

   Purpose

   Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   PP7-mVenus
   

   Use

   Synthetic Biology

   Tags

   HA, SV40 NLS, and mVenus

   Expression

   Mammalian

   Mutation

   PP7: C68S, S69G [delta]70-75 (non-aggregating)

   Promoter

   hUBC

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV.PGK.hLHX6

   Plasmid

   #161828

   Purpose

   Expresses human LHX6(LIM Homeobox 6) in mammalian cells

   Depositor

   Daniella Ottosson

   Article

   Giacomoni et al Cells. 2020 Nov 10;9(11). pii: cells9112

   Insert

   human LHX6 (LHX6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   AA 17-23 deletion

   Promoter

   PGK

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ALC1-c007

   Plasmid

   #173112

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   CHD1L (CHD1L Human)
   

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   L613-K879, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HsCLYBL-FLAG

   Plasmid

   #111290

   Purpose

   mammalian expression

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   human CLYBL (CLYBL Human)
   

   Tags

   linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

   Expression

   Mammalian

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. HsCLYBL(D320A)-FLAG

   Plasmid

   #111291

   Purpose

   mammalian expression

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   human CLYBL D320A (CLYBL Human)
   

   Tags

   linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

   Expression

   Mammalian

   Mutation

   D320A

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP C3-YAP-deltaC

   Plasmid

   #21126

   Depositor

   Marius Sudol

   Article

   Tsutomu Oka & Marius et al

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Tags

   EGFP-C3

   Expression

   Bacterial and Mammalian

   Mutation

   5 Carboxy-terminal amino acids -FLTWL are deleted

   Available Since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI

    Plasmid

    #192112

    Purpose

    Expresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Insert

    SARS-CoV-2-S2P-BA.4/5 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. px458_2A_GFP_sgRNA_G3BP2_G1

    Plasmid

    #127112

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA G3BP2 (G3BP2 Human)
    

    Use

    CRISPR

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cry2PHR.mCh.CofS3E

    Plasmid

    #133619

    Purpose

    Expresses Cry2PHR - mCherry - CofilinS3E protein fusion

    Depositor

    Robert Hughes

    Article

    Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

    Insert

    Cry2PHR - mCherry - CofilinS3E
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-sgRNA3-RPB1-N-term

    Plasmid

    #195112

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pInducer10-UBC9-sh4

    Plasmid

    #168988

    Purpose

    Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Ubiquitin Conjugating Enzyme 9
    

    Expression

    Mammalian

    Promoter

    Ubc promoter

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIRECT_21B

    Plasmid

    #91129

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-30a(+)-Syk-tSH2-PM

    Plasmid

    #111271

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    

    Expression

    Bacterial

    Mutation

    Y130E mutation to mimic phosphorylation

    Promoter

    T7 promoter

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAD/CMV/V5-DEST-Rac1-2G

    Plasmid

    #66112

    Purpose

    Second generation Rac1 FRET biosensor for adenovirus production

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Adenoviral

    Tags

    His-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Actin.CIB.GFP

    Plasmid

    #133618

    Purpose

    Expresses beta actin - CIBN - GFP protein fusion

    Depositor

    Robert Hughes

    Article

    Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

    Insert

    betaActin - CIB
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. ActinS14V.CIB.GFP

    Plasmid

    #133620

    Purpose

    Expresses beta actin S14V - CIBN - GFP protein fusion

    Depositor

    Robert Hughes

    Article

    Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

    Insert

    betaActin - CIB
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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