We narrowed to 24,600 results for: Nes

1. pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

   Plasmid

   #184645

   Purpose

   Retroviral expression of BirA Rap1 I312R

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   BirA-rap1-I312R
   

   Use

   Retroviral

   Tags

   BirA FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJCC_164 SpCas9 R1333A/R1335A

   Plasmid

   #179528

   Purpose

   For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 R1333A/R1335A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginines 1333 and 1335 to alanine

   Promoter

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMP71-mGFP-llo190-mc38minor

   Plasmid

   #174604

   Purpose

   expresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   palmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

   Plasmid

   #159664

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

   Plasmid

   #159663

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

   Plasmid

   #159662

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

   Plasmid

   #159656

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-dt/deltaEGFP

   Plasmid

   #163919

   Purpose

   PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   dtomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBV-luc TOP2A 500bp promoter

   Plasmid

   #138273

   Purpose

   Luciferase reporter plasmid driven by TOP2A promoter

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A promoter sequence
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CAG.LSL.tdTomato

    Plasmid

    #100048

    Purpose

    AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdtomato
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmGFP-ADAR1-p150

    Plasmid

    #117927

    Purpose

    ADAR1-p150 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p150 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGES201

    Plasmid

    #190197

    Purpose

    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMP1

    Plasmid

    #79873

    Purpose

    Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

    Depositor

    Jason Peters, Carol Gross, Stanley Qi

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    xylA

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Cre-T2A-mRuby2

    Plasmid

    #102989

    Purpose

    Expresses Cre and mRuby2

    Depositor

    Karin Scharffetter-Kochanek

    Article

    Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

    Inserts

    Cre (site-specific recombinase)
    mRuby2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-HA-Ubiquitin-K33

    Plasmid

    #17607

    Purpose

    Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K33 only, other lysines mutated to arginines; P19…

    Promoter

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmGFP-ADAR1-p110

    Plasmid

    #117928

    Purpose

    ADAR1-p110 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p110 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBa.GFP-KIF13A tail 361-1749

    Plasmid

    #134622

    Purpose

    Mammalian expression of mouse KIF13A 361-1749

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13A (Kif13a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 360 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBa.GFP-KIF13B tail 442-1843

    Plasmid

    #134623

    Purpose

    Mammalian expression of mouse KIF13B 442-1843

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13B (Kif13b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 441 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pG3H-PE3max-attR1R2

    Plasmid

    #213049

    Purpose

    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Inserts

    Cas9 nickase
    M-MLV Reverse Transcriptase
    

    Use

    Note: this plasmid needs helper pvs1-vir2 for rep…

    Tags

    Expression

    Plant

    Mutation

    H840A, R240K, N413K,

    Promoter

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLCKO

    Plasmid

    #73311

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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