We narrowed to 15,037 results for: pET

1. pFA6a-link-ymYPET-URA3

   Plasmid

   #168056

   Purpose

   Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Use

   Tags

   ymYPET

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MBP-CBX1-eReader

   Plasmid

   #135089

   Purpose

   Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-2xMreBN-mCherry-His

   Plasmid

   #133626

   Purpose

   Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   2xMreB-mCherry-His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SARS-CoV-2 NSP13

   Plasmid

   #176492

   Purpose

   Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

   Depositor

   Xiaodong Cheng

   Article

   White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

   Insert

   SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   I570 from SARS-CoV NSP13 was mutated to V to conv…

   Promoter

   T7

   Available Since

   Jan. 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET22-Nsp10-TEV-6His

   Plasmid

   #154957

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   TEV-6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   July 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET-Sac-Abeta(MC1-40)

   Plasmid

   #127152

   Purpose

   Expresses N-terminal cysteine Abeta(1-40)

   Depositor

   James Nowick

   Insert

   Abeta(MC1-40) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. His6-SUMO-pET28a-EfaCas1

   Plasmid

   #112793

   Purpose

   To express enterococcus faecalis Cas1 protein in E. coli

   Depositor

   Ailong Ke

   Article

   Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

   Insert

   EfaCas1
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-GFP-AURKA_K162M-mCherry

   Plasmid

   #99881

   Purpose

   Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Mutation

   K162M (kinase-dead)

   Promoter

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mucin MUC1-5TR-pET15b

   Plasmid

   #174694

   Purpose

   Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

   Depositor

   Jean Gariepy

   Article

   Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

   Insert

   MUC1-TR
   

   Use

   Tags

   hexahistidine (HIS) tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 Promoter

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-SUMO-hDDI2-D252N_pET11a

    Plasmid

    #169122

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D252N

    Promoter

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-6xHis-(pos28)GFP

    Plasmid

    #89250

    Purpose

    Expresses GFP with +28 theoretical charge in E.Coli

    Depositor

    David Liu

    Insert

    His6-(pos16)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET 15b-UbcH5a/2417

    Plasmid

    #11472

    Depositor

    Allan Weissman

    Article

    Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

    Insert

    UbcH5a (UBE2D1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pET22b-mEB1-eGFP-6xHis

    Plasmid

    #130981

    Purpose

    Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB1-GFP-6xHis (Mapre1 Mouse)
    

    Use

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-51b(+)-cpHaloTag141/145

    Plasmid

    #194660

    Purpose

    Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHaloTag141/145
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-51b(+)-cpHaloTag154/156

    Plasmid

    #194661

    Purpose

    Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHaloTag154/156
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28_mouse-Caspase-11_enzymatic-domain

    Plasmid

    #183382

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-11 (AA100-373) (Casp4 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET16b-Rnl2(1-249)K227Q

    Plasmid

    #14072

    Depositor

    Thomas Tuschl

    Insert

    Rnl2(1-249)
    

    Use

    Tags

    His (10x)

    Expression

    Bacterial

    Mutation

    changed Lys227 to Glutamine Gly112 is actually C…

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

18. [pSK104] pET28 SLC38A9N-His

    Plasmid

    #136142

    Purpose

    bacterial expression of SLC38A9 N term fragment (1-119)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    SLC38A9 N term fragment (1-119) (SLC38A9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

    Plasmid

    #198750

    Purpose

    Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - SmallBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Use

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

    Plasmid

    #198751

    Purpose

    Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - LargeBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET16b-Trx CC32/35SS

    Plasmid

    #36946

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    both active site cysteines (32,35) mutated to ser…

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pF709-pET28a-Hs-FL-ETFbeta-NHis wt

    Plasmid

    #85110

    Purpose

    expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length ETFbeta (ETFB Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET30b-5M SrtA with TEV site

    Plasmid

    #86962

    Purpose

    Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

    Depositor

    Ron Bose

    Article

    Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

    Insert

    S.aureus SrtA 5M
    

    Use

    Tags

    6x Histidine tag and TEV site

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a(+) 6xHis-3xFlag-IFIT5

    Plasmid

    #53561

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Use

    Tags

    3xFLAG, 6xHIS, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pET_SZ1-RGG-GFP-RGG

    Plasmid

    #124937

    Purpose

    Expression of SZ1-RGG-GFP-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 3521 pET15b Bid p15

    Plasmid

    #8782

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

28. pET51b(+)_LAMA-F98 (for GFP)

    Plasmid

    #130714

    Purpose

    Plasmid encoding the bacterial expression of LAMA-F98 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-F98
    

    Use

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28-Nsp14-TEV-6His

    Plasmid

    #154952

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pET-Octo_VirD2_NLS-Cas9-6xHis

    Plasmid

    #232296

    Purpose

    Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

    Depositor

    Hee-Sik Kim

    Article

    Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

    Insert

    Octo_VirD2_NLS-Cas9-6xHis
    

    Use

    CRISPR

    Tags

    6xHis and Octopine-type VirD2-derived NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge