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We narrowed to 5,049 results for: Mos

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Showing: 4081 - 4120 of 5049 results

  2. Desmoplakin II acceptor only control (F40-based tension sensor)

    Plasmid
    #118719
    Purpose
    The acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)(Y67G)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118719-plasmid-map-sequence-id-230445

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  4. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92139-plasmid-map-sequence-id-349059

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  6. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177334-plasmid-map-sequence-id-348602

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  7. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

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  9. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  22. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    118155-plasmid-map-sequence-id-243704

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  23. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid
    #177335
    Purpose
    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177335-plasmid-map-sequence-id-348601

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Showing: 4081 - 4120 of 5049 results