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Plasmid#108315PurposeGFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.DepositorInsertGFP under control of arabinose-inducible promoter pBAD
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
SIVu
Plasmid#81089PurposeSingle Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-deltaOVA
Plasmid#64595PurposeExpression of N-terminally truncated chicken ovalbumin as model antigen for study of MHC class I and class II presentationDepositorInsertOvalbumin
UseTagsExpressionMammalianMutationLacking first 49 aa (leader sequence for transloc…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBTK1024 (pDS-Def1)
Plasmid#183125PurposeExpresses double-stranded RNA targeting the Def1 gene from Apis melliferaDepositorInsertFragment of Def1 gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
DS-SPcas
Plasmid#48645PurposeBacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
tracrRNA precursor
UseCRISPRTagsExpressionMutationPromoterproC and tracrRNA promoterAvailable sinceDec. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNC-Cam1304-MCS
Plasmid#193387PurposeNimble cloning-compatible vector for plant ectopic expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXW117Ptet2-gfp
Plasmid#160818PurposeaTc-responsive single-layered circuitDepositorInsertPJ23117-30tetR-t-Ptet2-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPJ23117, Ptet2Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-pgk-puro
Plasmid#85747Purposetetracyline-inducible lentiviral vector with no insert for use in mammalian cellsDepositorTypeEmpty backboneUseLentiviralTagsnoneExpressionMutationPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
NM1-5S-tRNA-SgH
Plasmid#128178PurposeExpresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoterDepositorInsertgRNA cloning cassette
UseCRISPRTagsExpressionYeastMutationPromoter5S rRNA-tRNA(Arg)Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-BsaI_pJMP2776
Plasmid#208890PurposeAcinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Ran(Q69L)
Plasmid#42048DepositorInsertRan (RAN Human)
UseTagsHisExpressionBacterialMutationQ69LPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK2-AraE
Plasmid#110141PurposeConstitutive promoter expressing araE on RK2 originDepositorInsertaraE
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-mPIAS1
Plasmid#15206DepositorInsertPIAS1 (Pias1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-GFP
Plasmid#35363DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSL1394
Plasmid#129522PurposepDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFLAG-NLSExpressionMutationPromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP8045
Plasmid#220900PurposeTn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroidesDepositorInserttnsABCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
SIVl
Plasmid#81090PurposeSingle Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p210 pCMV-CREM
Plasmid#8395PurposeCre with chimeric/β-globin intron within the Cre coding sequenceDepositorInsertCREM
UseCre/LoxTagsExpressionMammalianMutationmodified CrePromoterCMVAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
peGFP-XPO5
Plasmid#58331PurposeExpresses human XPO5 on the C-terminus of eGFPDepositorInsertExportin-5 (XPO5 Human)
UseTagsExpressionMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
PKC delta DN
Plasmid#16389DepositorInsertPKC delta isoform 2 (Prkcd Mouse)
UseTagsHAExpressionMammalianMutationDominant negative (kinase inactive): K376RPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBbA6c-RFP
Plasmid#35290DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLlacO-1Available sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJKR-O-mphR
Plasmid#62570PurposeMacrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM5057
Plasmid#120085PurposeBroad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.DepositorInsertRSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attR4-pegRNA-attR3
Plasmid#213056PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attL4
Plasmid#213055PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-LexA
Plasmid#26258DepositorInsertLexA
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
SIVt
Plasmid#81092PurposeSingle Integration Vector TRP - Cloning vector for single integration into the TRP1 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBR8k-GFPuv
Plasmid#106385PurposeL-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 Smad4
Plasmid#16483DepositorInsertSmad4 (SMAD4 Human)
UseTagsDouble HAExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKT-iRFP-KAN
Plasmid#64687PurposepKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.DepositorInsertiRFP
UseTagsExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…PromoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbE1a-RFP
Plasmid#35331DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTrcAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFS461
Plasmid#89064Purposeleu1 integration plasmid with adh1pr:Z3EVDepositorInsertZ3EV artificial transcription factor
UseLeu1 integrating vectorTagsExpressionMutationPromoteradh1Available sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDR1-1202
Plasmid#37627DepositorInsertMDR1 promoter (ABCB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_Helper
Plasmid#190432PurposeM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)DepositorInsertM13 genes: I-XI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_M13ps_LacZα_gIII
Plasmid#190435PurposeM13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.DepositorInsertsM13 packaging signal
M13 gIII
LacZα
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
SIVh
Plasmid#81091PurposeSingle Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW071
Plasmid#140037PurposePCC 11901 fadD locus KO with a kanamycin resistance cassetteDepositorInsertKanamycin resistance gene
UseGene knockout in cyanobacteriaTagsExpressionMutationPromoterKanRAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
NM9-SpCas9-NLS3
Plasmid#128177PurposeExpresses R. toruloides codon-optimized SpCas9 under PGK1 promoterDepositorInsertSpCas9-NLS3
UseCRISPRTagsExpressionYeastMutationPromoterpPGK1Available sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbB2k-GFP
Plasmid#35345DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTetAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pBBR6k-J23101-GFPuv
Plasmid#106390PurposepBBR6k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterLlacO-1Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR hH1-TERRA
Plasmid#111381PurposeTERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminatorDepositorInserthH1-TERRA
UseGateway entry cloningTagsExpressionMutationPromoterhH1Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX6-FlagTag (pcDNA3.1+)
Plasmid#75217PurposeMammalian expression of SLC8B1DepositorInsertSLC8B1 (SLC8B1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKC beta I DN
Plasmid#16381DepositorInsertPKC beta 1 (Prkcb Rat)
UseTagsHAExpressionMammalianMutationDominant negative (kinase inactive): K371RPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-10XGP41-J9
Plasmid#202005PurposeMoClo level 0 dCas9-10XGP41 coding sequence moduleDepositorInsertdCas9-10XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-TEAD1-Y406A
Plasmid#33047DepositorInsertTEAD1 (TEAD1 Human)
UseTagsMycExpressionMammalianMutationY406APromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBPGAL4.2::p65Uw
Plasmid#26229DepositorInsertGAL4::p65 (RELA Budding Yeast, Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-cdaR
Plasmid#62557PurposeGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.DepositorInsertsbeta lactamase
sfGFP
CdaR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-tdMCP-24xGCN4
Plasmid#205157Purposeexpress tdMCP-24xGCN4DepositorInserttdMCP-24xGCN4
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only