We narrowed to 165,567 results for: addgene

1. p184_LTJ_sgRNACD90.2

   Plasmid

   #82670

   Purpose

   sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting mouse CD90.2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FUG+p97W (C2)

   Plasmid

   #74018

   Purpose

   lentiviral expression of EGFP-SAP97beta fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   SAP97 beta isoform
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiMutB3GNT5-blast

   Plasmid

   #208380

   Purpose

   lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nt 922 c->g (PAM site inactivation)

   Promoter

   EF-1 alpha core promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDB4281

   Plasmid

   #98700

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 53BP1 N-terminal sgRNA

   Plasmid

   #207091

   Purpose

   pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GGGGAGCAGATGGACCCTAC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPR_eGFP_497

   Plasmid

   #111824

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 477-497
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p-dCas9-LSD1-K661A-Hygro

   Plasmid

   #104414

   Purpose

   transient expression of dCas9-dLSD1 fusion protein

   Depositor

   Stephan Beck

   Insert

   dLSD1 (KDM1A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   IRATp970A0364D (Source Bioscience) has a P405H ch…

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ATG5 sgRNA

   Plasmid

   #207555

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG5 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   AACTTGTTTCACGCTATATC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLJM1-PCYT2

   Plasmid

   #81074

   Purpose

   Expresses PCYT2 in mammalian cells

   Depositor

   Erin O'Shea

   Article

   Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

   Insert

   phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. M4755 KanMX::LEU2 Disruptor Converter

    Plasmid

    #51685

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to LEU2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LEU2 (LEU2 Budding Yeast)
    

    Use

    Yeast marker swap

    Expression

    Yeast

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ZAK (ZAKA-c057)

    Plasmid

    #98225

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ZAKA (MAP3K20 Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    pfam00069:Pkinase 16-257

    Promoter

    polyhedrin

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Id2 Ngfr

    Plasmid

    #80131

    Purpose

    overexpression of ID2

    Depositor

    Ellen Rothenberg

    Article

    Del Real et al Development. 2013 Mar;140(6):1207-19. do

    Insert

    Id2 (Id2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-Ngfr

    Expression

    Mammalian

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ULK1 KO sgRNA

    Plasmid

    #207561

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CCCGCCTGCGCCATGGAGCC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEVY-GA

    Plasmid

    #51227

    Purpose

    bi-directional expression vector for yeast, galactose inducible, ADE2 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GAL1,10

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR_eGFP_191

    Plasmid

    #111821

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 171-191
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pI6-MTU-DO-URA

    Plasmid

    #160030

    Purpose

    Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG306-GPD-AVT1 chr I

    Plasmid

    #41898

    Purpose

    Yeast expression of AVT1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    AVT1 (AVT1 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    GPD

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBabe NEO Venus

    Plasmid

    #98396

    Purpose

    Retroviral vector for constitutive Venus expression

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Venus
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVU-tTR-KRAB

    Plasmid

    #11645

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, no tetO
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEpic

    Plasmid

    #84372

    Purpose

    3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge