We narrowed to 12,455 results for: cel.2

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pCMV6-AN-DDK_1-590 POLI

Plasmid

#131233

Purpose

Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_1-420 POLI

Plasmid

#131234

Purpose

Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-TEV-Nrf2

Plasmid

#62455

Purpose

Halo-His6 tagged Nrf2 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Nrf2 (NFE2L2 Human)

Tags

His6-Halo-TEV

Expression

Bacterial

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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SMC2 CRISPR

Plasmid

#140649

Purpose

SMC2 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

SMC2 (SMC2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-CRY2-VHH(GFP)

Plasmid

#58370

Purpose

For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Tags

SNAP and vhhGFP4

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pMC-cIAP1-FL

Plasmid

#20209

Depositor

Martin Holcik

Article

Zhao et al Cell Death Differ. 2009 Feb . 16(2):244-

Insert

cIAP1 3'UTR (BIRC2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
p5E-Runx1+23

Plasmid

#69602

Purpose

Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

Depositor

Owen Tamplin, Leonard Zon

Article

Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

Inserts

Runx1 +23 intronic enhancer (Runx1 Mouse)
Mouse beta-globin minimal promoter

Use

5' gateway entry vector

Promoter

C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPBbleo-ErbB2

Plasmid

#197361

Purpose

Encoding human ErbB2.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

ERBB2 (ERBB2 Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi G418 Hu Her2

Plasmid

#183246

Purpose

Expression of human HER2 antigen in a Sleeping Beauty transposon vector

Depositor

Alexander McLellan

Article

Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

Insert

Her2 (ERBB2 Human)

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

Plasmid

#192939

Purpose

FlpO-based digitizer circuit driven by AP1 promoter

Depositor

Wilson Wong

Article

Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:

Inserts

AP1(2)/minTK promoter
FlpO-ABI
PYL-FlpO
CAG promoter
frt-STOP-frt
GFP
luciferase
pPGK
Zeocin resistance
BFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP/golgin-160/3DE

Plasmid

#180631

Purpose

Expression of caspase-resistant golgin-160

Depositor

Carolyn Machamer

Article

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160/3DE (GOLGA3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D59E, D139E, D311E

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHR KIF17 (1-738)

Plasmid

#60406

Purpose

Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

KIF17 (KIF17 Human)

Use

Lentiviral

Tags

GB1, Strep-Tag, and sfGFP

Expression

Mammalian

Mutation

Truncated version amino acids 1-738

Promoter

SFFV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA Ago2

Plasmid

#10821

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 2 (AGO2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

R668H. Additionally, N terminus different from wi…

Available Since

Oct. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-Cterm-V5His6_H

Plasmid

#146488

Purpose

Insect Expression of DmPABPC1-Cterm

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-Cterm (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-Nterm-V5His6_H

Plasmid

#146489

Purpose

Insect Expression of DmPABPC1-Nterm

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-Nterm (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmPABPC1-Nterm-V5His6_H

Plasmid

#146478

Purpose

Insect Expression of DmPABPC1-Nterm

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-Nterm (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GABA(A) receptor subunit a2SE

Plasmid

#49169

Purpose

pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

Depositor

Tija Jacob, Stephen Moss

Article

Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

Insert

GABA(A) receptor subunit alpha-2 (GABRA2 Human)

Tags

HA, bovine alpha 1 signal sequence, and pHGFP (pH…

Expression

Mammalian

Mutation

T343A

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt2

Plasmid

#220245

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2-Fc(IgG1)

Plasmid

#145165

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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