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We narrowed to 59,686 results for: plasmid

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Showing: 2041 - 2060 of 59686 results

  2. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid
    #55201
    Purpose
    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55201-plasmid-map-sequence-id-240663

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  3. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Publication
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60490-plasmid-map-sequence-id-332535

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  7. BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A

    Plasmid
    #101192
    Purpose
    Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K918A/V922A/R925A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101192-plasmid-map-sequence-id-194255

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  8. MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A

    Plasmid
    #101188
    Purpose
    Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    T657A/G658A/W659A/R661A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101188-plasmid-map-sequence-id-194251

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  9. MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A

    Plasmid
    #101190
    Purpose
    Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    F491A/M495A/T496A/N497A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101190-plasmid-map-sequence-id-194253

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  10. MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A

    Plasmid
    #101184
    Purpose
    Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Mutation
    G582A/V583A/E584A/D585A/N588A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101184-plasmid-map-sequence-id-194248

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  11. MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A

    Plasmid
    #101179
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101179-plasmid-map-sequence-id-194003

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  15. TRMPVIR

    Plasmid
    #27994
    Purpose
    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
    Depositor
    Scott Lowe
    Publication
    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.
    Insert
    TRMPVIR (shRen)
    Use
    RNAi and Retroviral; Fluorescent reporters
    Expression
    Mammalian
    Available since
    March 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    27994-plasmid-map-sequence-id-13249

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  16. pEditA

    Plasmid
    #207531
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Publication
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207531-plasmid-map-sequence-id-409606

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  17. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50463
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50463-plasmid-map-sequence-id-75416

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  18. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid
    #196702
    Purpose
    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.
    Depositor
    Karlene Cimprich
    Publication
    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human RNaseH1 wild type
    Expression
    Mammalian
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196702-plasmid-map-sequence-id-385953

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  20. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185912-plasmid-map-sequence-id-367362

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Showing: 2041 - 2060 of 59686 results