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pAG303-Gal-PR50

Plasmid

#84905

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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CoV2-M-IRES-E

Plasmid

#177938

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )

Expression

Mammalian

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Q-IL6-9

Plasmid

#166552

Purpose

scFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptide

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, Sortag, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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CARPID BASU-dCasRx

Plasmid

#153209

Purpose

express CARPID BASU-dCasRx fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Article

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

BASU-dCasRx

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

Plasmid

#205565

Purpose

Express human NEDD4L (delta C2 isoform)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

NEDD4L (NEDD4L Human)

Use

Piggybac

Expression

Mammalian

Mutation

delta C2 isoform

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-M3M14nes

Plasmid

#155367

Purpose

Targeted m6A RNA methylation in mammalian cells

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)

Use

CRISPR

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDT-sgRNA

Plasmid

#138271

Purpose

Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

Depositor

Xiao Wang

Article

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

sgBG, sgTS

Use

CRISPR

Expression

Mammalian

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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U6GRNA

Plasmid

#68370

Purpose

gRNA scaffold with hU6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC7A3_STOP

Plasmid

#161467

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A3 (SLC7A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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