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pIRES-hrGFP II-mTET1

Plasmid

#83569

Purpose

Expresses TET1 with a mutated catalytic domain in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

catalytic domain mutant H1672D, D1674A

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp1

Plasmid

#72687

Purpose

Doxycyline regulated expression of Mesp1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 1 (MESP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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CDK9_pLX307

Plasmid

#98324

Purpose

Lentiviral expression of CDK9

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

CDK9 (CDK9 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pUC57-GFP-SFPQ-RT

Plasmid

#97090

Purpose

Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

EGFP-SFPQ-RT (SFPQ Human)

Use

CRISPR

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1m_v1-RT

Plasmid

#97089

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1m_v1-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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OptoEphB2-mCherry

Plasmid

#92042

Purpose

Expresses OptoEphB2 labeled with mCherry

Depositor

Ji Yu

Insert

EPHB2 (EPHB2, CRY2 Human, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-Caveolin1-CMV-GFP

Plasmid

#83272

Purpose

Adenoviral expression of Caveolin1 with co-expression of GFP

Depositor

Andrew Brooks

Article

Fernandez-Rojo et al Cell Rep. 2013 Jul 25;4(2):238-47. doi:

Inserts

CAV1 (CAV1 Human)
eGFP

Use

Adenoviral

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alu-Lin28

Plasmid

#92346

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived Alu element containing 3'UTR (LIN28A Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Article

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-FOXM1-shRNA-84

Plasmid

#89494

Purpose

Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

Depositor

Adam Karpf

Insert

FOXM1 (FOXM1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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