We narrowed to 2,800 results for: rela

1. pCAGGS-SMS2-TEV-Twin-Strep

   Plasmid

   #202529

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS2 (SGMS2 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TGA)

   Promoter

   CAG and chicken β-actin promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-2NLS-Cas9-6XMS2

   Plasmid

   #166033

   Purpose

   Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiGuide-Hygro-dTomato

   Plasmid

   #99376

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-dTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHis-BRD4 BD1

   Plasmid

   #196544

   Purpose

   Expression of BRD4 Bromodomain (BD1) in E.coli

   Depositor

   Byung Il Lee

   Article

   Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

   Insert

   BRD4 BD1 (BRD4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-Hygro-eGFP

   Plasmid

   #99375

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRD405

   Plasmid

   #163110

   Purpose

   Expression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNA

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS_mScarlet-I_H-NSdbd_NLS
   

   Use

   Eukaryotic expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter; T7 promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMYH9-D1424H-FLAG

   Plasmid

   #183513

   Purpose

   Gateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.

   Depositor

   Emanuele Panza

   Article

   Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

   Insert

   MYH9 (MYH9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Aspartic acid 1424 to Histidine

   Promoter

   CMV

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-EF1a-dCas9-VP64-Puro

   Plasmid

   #99371

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-VP64-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

   Plasmid

   #193728

   Purpose

   Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

   Depositor

   Colin Akerman

   Article

   Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

   Insert

   ClopHensorN
   

   Use

   AAV

   Tags

   Strep-II (N terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX62

    Plasmid

    #46854

    Purpose

    TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

    Depositor

    Monica Hollstein

    Article

    Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

    Insert

    attB-Puro-2A and Hupki p53 G245S (TP53 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G245S

    Promoter

    Available since

    Aug. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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