We narrowed to 4,533 results for: ARA-2

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pPHR-mCherry-mHP1alpha-I163A

Plasmid

#181889

Purpose

Soluble light-dependent effector protein (HP1alpha with I163A mutation) for optodroplet formation or recruitment to CIBN localizers,

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-mHP1alpha-I163A (Cbx5 Mouse, Mustard Weed)

Tags

PHR-mCherry

Expression

Mammalian

Mutation

I163A

Promoter

CMV

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pISH-Vmn2r76

Plasmid

#108084

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r76 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r76 (Vmn2r76 Mouse)

Use

In vitro transcription

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAdEasy-Msln-iCre-HA-Flag

Plasmid

#31305

Depositor

William Pu

Article

Zhou et al J Clin Invest. 2011 May 2. 121(5):1894-9

Insert

Mesothelin (MSLN Human)

Use

Adenoviral

Tags

Flag and HA

Available Since

March 1, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-AAV2cap

Plasmid

#198014

Purpose

For high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM library

Depositor

George Church

Article

Jain et al Elife. 2024 Mar 27;12:RP87730. doi: 10.7

Insert

AAV2 cap

Use

AAV

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pN7HELIX

Plasmid

#190662

Purpose

Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB

Expression

Mammalian

Mutation

nAniI = K227M, F80K, L232K

Promoter

CMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pN7CAST

Plasmid

#190661

Purpose

Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-HA-COPS6

Plasmid

#22542

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

COPS6 (COPS6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pISH-Vmn2r65

Plasmid

#108083

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r65 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r65 (Vmn2r65 Mouse)

Use

In vitro transcription

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Vmn2r76-VR2

Plasmid

#108085

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r76 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r76 (Vmn2r76 Mouse)

Use

In vitro transcription

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3/er-(n2)oxStayGold(c4)v2.0

Plasmid

#186296

Purpose

A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

er-(n2)oxStayGold(c4)v2.0

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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HA-SOCS1

Plasmid

#174571

Purpose

Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

HA-SOCS1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-TEX264-deltaLRR-His6

Plasmid

#220215

Purpose

Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

His6

Expression

Bacterial

Mutation

c.4-75del / p.2-24del

Promoter

T7

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-FL

Plasmid

#128415

Purpose

Express INTS3 full-length in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

full-length

Promoter

tac promoter

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN2-pLenti6

Plasmid

#36950

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN2 (EGLN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN3-pLenti6

Plasmid

#36951

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN3 (EGLN3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pTwist-WIV1-CoV

Plasmid

#164438

Purpose

Encodes WIV1-CoV Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

WIV1-CoV Spike

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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iBE3 CLYBL

Plasmid

#174569

Purpose

doxycycline inducible BE3 expression for human CLYBL locus knock-in

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

BE3

Expression

Mammalian

Promoter

tet ON

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-N

Plasmid

#128416

Purpose

Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal region (1–513 aa)

Promoter

tac promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Hygro-mTagBFP2

Plasmid

#99374

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

Plasmid

#155305

Purpose

Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d

Use

Lentiviral

Tags

2xNLS-3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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