We narrowed to 15,384 results for: pET
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Plasmid#73421PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.DepositorInsertPromoter 4F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP4F2 (orthogonal T7-lac variant)Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET26-Ufd1-His
Plasmid#21266DepositorAvailable SinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla 3A
Plasmid#84872PurposeBacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
Fla
TagsHisExpressionBacterialMutation3 C-terminal leucines have been replaced by alani…PromoterT7Available SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)
Plasmid#199160Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+6)
TagsMGHHHHHHGGAMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM34
Plasmid#89156PurposeRecombinant expression of PTBP1-RRM34 in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationcontains RNA Recognition Motif 3+4PromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
LHP2691 - pET30-Ub-K63A
Plasmid#32179DepositorTagsHISExpressionBacterialMutationK63APromoterT7Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
LHP2690 - pET30-Ub-K48A
Plasmid#32178DepositorTagsHISExpressionBacterialMutationK48APromoterT7Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-6
Plasmid#172741PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-6
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-51b-HaloTag10
Plasmid#175520PurposeExpression of HaloTag10 in bacterial cellsDepositorInsertHaloTag10
TagsHisExpressionBacterialMutationHaloTag10 = HaloTag7-Q165WAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo2
Plasmid#25102DepositorAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_DeltaN24
Plasmid#214327PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
TagsHis-TEVExpressionBacterialMutationDeletion of first 24 amino acidsPromoterT7Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-5-enzymatic-domain
Plasmid#214306PurposeProtein overexpression in bacteriaDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Ypet-PBD
Plasmid#24533DepositorAvailable SinceAug. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-cpLuc2
Plasmid#135629PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)DepositorInsertNAD-cpLuc2 sensor
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-sfGO-Matryoshka-LE-LP
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29b-Ava3858-3856
Plasmid#45745DepositorInsertAva3858-3856
ExpressionBacterialPromoterT7Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET42a-lambdaN+-L+-GSH
Plasmid#98894PurposeExpresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo methodDepositorInsertlambdaN+-L+
TagsGST tagExpressionBacterialPromoterT7 promoterAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only