19,627 results

1. pMRX-INU-FLAG-FAM134B

   Plasmid

   #128260

   Purpose

   Expresses FAM134B in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   FAM134B (RETREG1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1.Gαs-LgB113

   Plasmid

   #134362

   Purpose

   Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαs-LgB113 (GNAS Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC013 - Twinstrep-SUMO-huLwCas13a

   Plasmid

   #90097

   Purpose

   Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   6xHis-Twin Strep-SUMO

   Expression

   Bacterial

   Available Since

   June 13, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLEX-EF1a ANXA11-mEmerald

   Plasmid

   #164210

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Promoter

   EF1a

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tet-O-FUW-Neurod1

   Plasmid

   #30129

   Depositor

   Marius Wernig

   Article

   Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

   Insert

   NeuroD1 (Neurod1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYIC

   Plasmid

   #18673

   Depositor

   Han Htun

   Article

   Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

   Type

   Empty backbone

   Tags

   3Myc-EYFP-HA-His6 and ECFP-HA-His6

   Expression

   Mammalian

   Available Since

   Aug. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TRE3G-MYCN

   Plasmid

   #104542

   Purpose

   PiggyBac vector encoding doxycycline-inducible human MYCN

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
   

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3G

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. nls-EGFP

   Plasmid

   #67652

   Purpose

   Mammalian expression vector driving nls-EGFP.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   nls-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCX419

   Plasmid

   #229216

   Purpose

   All-in-one plasmid for labeling of XIST RNA via Csm complex

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA
   

   Expression

   Mammalian

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rogi1-CMV-LAMB3-T2A-GFP-bGH

    Plasmid

    #179864

    Purpose

    Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

    Depositor

    George Church

    Article

    Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

    Insert

    LAMB3-T2A-GFP
    

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mscv2.2-NLRC4

    Plasmid

    #60199

    Purpose

    Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-KG-D4H*-mCherry

    Plasmid

    #134604

    Purpose

    fluorescent cholesterol-binding probe

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    D4H*
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    Y415A and A463W

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TetO-FUW-NICD

    Plasmid

    #61540

    Purpose

    Expresses NOTCH-ICD in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    NOTCH-ICD (NOTCH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NOTCH intracellular domain (aa1761-2555); A1943S…

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV4-ApoE2

    Plasmid

    #87085

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E2 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

    Plasmid

    #162278

    Purpose

    Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

    Depositor

    Al George

    Article

    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

    Insert

    SCN1A Variant 1, stabilized (SCN1A Human)
    

    Tags

    IRES-mScarlet

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp2946. Silent codon c…

    Promoter

    CMV

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti MS2-P65-HSF1_Hygro

    Plasmid

    #61426

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB CAG-sfGFP

    Plasmid

    #133568

    Purpose

    CAG sfGFP piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CMV-puro-2A-TEVp

    Plasmid

    #99610

    Purpose

    Lentivirus expressing puromycin resistance gene and TEV protease

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    Puromycin resistance (PAC gene)
    TEV protease
    

    Use

    Lentiviral

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115219

    Purpose

    Expresses PspCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PspCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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