We narrowed to 4,857 results for: Mela

1. pAc5.1B-EGFP-DmTral-F44A_C

   Plasmid

   #146044

   Purpose

   Insect Expression of DmTral-F44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-F44A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two silent mutations A198C, A387G and a mutation …

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-EGFP-DmTral-K73AD74A_C

   Plasmid

   #146045

   Purpose

   Insect Expression of DmTral-K73AD74A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-K73AD74A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two silent mutations A198C, A387G and a mutation …

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pnCS_mEGFP-DSep2_Peanut-Strep

   Plasmid

   #174496

   Purpose

   bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut

   Depositor

   Manos Mavrakis

   Article

   Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi

   Inserts

   Sep2 (Sep2 Fly)
   peanut (pnut Fly)
   

   Use

   Tags

   Strep and mEGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-DEST42-dre4

   Plasmid

   #170440

   Purpose

   Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   dre4 (dre4 Fly)
   

   Use

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-20

   Promoter

   T7

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-EGFP-DmTral-R42E_B

   Plasmid

   #145988

   Purpose

   Insect Expression of DmTral-R42E

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42E (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two silent mutations A198C, A387G and a mutation …

   Promoter

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDEST-Hemmar-DSCP-ftz-FLP(D)

   Plasmid

   #119894

   Purpose

   FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

   Depositor

   Rita Sousa-Nunes

   Article

   Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

   Inserts

   FLP(D)
   DSCP-ftz (ftz Fly)
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   a mutated form of FLP, which at position 5 contai…

   Promoter

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D

   Plasmid

   #112986

   Purpose

   P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331D) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   T331D

   Promoter

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A

   Plasmid

   #112988

   Purpose

   P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331A) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   T331A

   Promoter

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAL c2x Tral N

   Plasmid

   #113009

   Purpose

   For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   N terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amino acids 1-355

   Promoter

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMAL c2x Tral FL-15A

    Plasmid

    #113011

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to A

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMAL c2x Tral C

    Plasmid

    #113008

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acids 356-652

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMAL c2x Tral C 15A

    Plasmid

    #113007

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PNG phosphorylation sites mutated to A

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFOSGE_COF4D03

    Plasmid

    #70969

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf12 (Taf12 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pFOSGE_CUS1F03

    Plasmid

    #71071

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Su(var)205 (Su(var)205 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pUAST-HA, Pbl-A C-Term

    Plasmid

    #69795

    Purpose

    Pebble isoform A, C-Term, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    van Impel et al Development. 2009 Mar;136(5):813-22. doi

    Insert

    Pebble C-Term domain (Drosophila guanine nucleotide exchange factor)
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA-Tag

    Expression

    Insect

    Mutation

    Just the C-Term domain of Pebble (pbl)

    Promoter

    hsp70 promoter

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

16. KHBD00295

    Plasmid

    #34421

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG12052 (lola Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. KHBD00321

    Plasmid

    #34446

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8676 (Hr39 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

18. KHBD00050

    Plasmid

    #34212

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG11502 (svp Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. KHBD00060

    Plasmid

    #34221

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9415 (Xbp1 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. KHBD00012

    Plasmid

    #34174

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8328 (HLHmdelta Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pCMV-Tag4A-MMP8 (E87D)

    Plasmid

    #29547

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    E87D

    Promoter

    Available Since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pCDF1-ADAM29 (E111K)

    Plasmid

    #31144

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Glutamic acid 111 changed to Lysine

    Promoter

    Available Since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

23. PCMV-Tag4A-MMP8 (E138Q)

    Plasmid

    #29549

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    E138Q

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pCDF1-ADAMTS18 (Q1002X)

    Plasmid

    #29557

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glutamine 1002 to stop codon

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pCDF1-ADAMTS18 (Q904X)

    Plasmid

    #29556

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glutamine 904 to stop codon

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pCDF1-ADAMTS18 (G312E)

    Plasmid

    #29553

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glycine 312 to glutamic acid

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pX330 p53

    Plasmid

    #59910

    Purpose

    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting p53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX330 Pten

    Plasmid

    #59909

    Purpose

    pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Pten (Pten Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

    Plasmid

    #106171

    Purpose

    cre mediated red to green shift

    Depositor

    Ewa Snaar-Jagalska

    Article

    Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

    Inserts

    dsRED-express2
    eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHIV-BRAFV600E-mOrange2

    Plasmid

    #110732

    Purpose

    Mammalian Expression of BRAFV600E IRES H2B-mOrange2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    BRAFV600E IRES H2B-mOrange2 (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    BRAF V600E

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pX330 Ctnnb1.1

    Plasmid

    #59911

    Purpose

    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLOC-MCOLN1

    Plasmid

    #131583

    Purpose

    For expression of human MCOLN1 in mammalian cells, GFP-tagged

    Depositor

    Sean Morrison

    Article

    Kasitinon et al Cell Rep. 2019 Aug 27;28(9):2293-2305.e9

    Insert

    MCOLN1 (MCOLN1 Human)
    

    Use

    Lentiviral

    Tags

    turboGFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. W45A mutant of H3K9me3 biosensor

    Plasmid

    #120808

    Purpose

    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCasper4-g-osk-TRICK

    Plasmid

    #71244

    Purpose

    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    oskar (osk Fly)
    

    Use

    P element-mediated germline transformation

    Tags

    12xPP7 and 6xMS2

    Expression

    Insect

    Mutation

    Promoter

    osk

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMP71-hPDL-1

    Plasmid

    #118631

    Purpose

    human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid

    Depositor

    Sébastien Walchli

    Article

    Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

    Insert

    hPDL-1 (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHIV-INK4A-mOrange2

    Plasmid

    #110730

    Purpose

    Mammalian Expression of p16INK4A IRES H2B-mOrange2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    p16INK4A IRES H2B-mOrange2 (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti6/V5-DEST-RNF2

    Plasmid

    #31216

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    RNF2 (RNF2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pBABE-hygro-2xMyc-Rac1-WT

    Plasmid

    #128580

    Purpose

    For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. K9L mutant of H3K9me3 biosensor

    Plasmid

    #120807

    Purpose

    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    lysine 9 is mutated to leucine 9 on histone H3

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEN35-CDKN2A-Ex2-R

    Plasmid

    #110737

    Purpose

    Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    CDKN2A gRNA (CDKN2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge