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We narrowed to 18,759 results for: nls

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Showing: 4121 - 4160 of 18759 results

  6. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177344
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter
    Depositor
    Naoki Yamamoto
    Publication
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Tetracycline-dependent promoters
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177344-plasmid-map-sequence-id-353629

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  8. pMIIS1011

    Plasmid
    #238212
    Purpose
    Expresses ParTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.
    Depositor
    Feng Zhang
    Publication
    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ParTasR
    Tags
    HA-NLS
    Expression
    Mammalian
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    238212-plasmid-map-sequence-id-471745

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  9. pLH_Scr15

    Plasmid
    #100537
    Purpose
    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Publication
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    N-terminal version of PhyB (AA 1-621)/ Mutation o…
    Promoter
    FBA1 and TDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100537-plasmid-map-sequence-id-194456

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  10. pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid
    #159856
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Publication
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    Expression
    Worm
    Mutation
    Not applicable
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159856-plasmid-map-sequence-id-318019

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  11. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  13. pCR1056

    Plasmid
    #71824
    Purpose
    Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS
    Depositor
    Jacob Corn
    Publication
    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
    Use
    CRISPR
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71824-plasmid-map-sequence-id-131017

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  14. pLenti-D156R LbCas12a-8xNLS

    Plasmid
    #248896
    Purpose
    Lentiviral vector expressing human codon-optimized D156R LbCas12a with 8xNLS
    Depositor
    Michael Aregger
    Insert
    D156R-LbCas12a-8xNLS-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248896-plasmid-map-sequence-id-498938

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  15. pLenti-hyper LbCas12a-8xNLS

    Plasmid
    #248897
    Purpose
    Lentiviral vector expressing human codon-optimized hyper LbCas12a with 8xNLS
    Depositor
    Michael Aregger
    Insert
    hyper-LbCas12a-8xNLS-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R/D235R/E292R/D350R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248897-plasmid-map-sequence-id-498940

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  16. plenti-ALFA-SpdCas9-2xNLS

    Plasmid
    #248898
    Purpose
    Lentiviral vector expressing human codon-optimized, ALFA-tagged SpdCas9 (deactivated DNAse domain)
    Depositor
    Michael Aregger
    Insert
    5xALFA-FLAG-SpdCas9-2xNLS-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/H840A
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248898-plasmid-map-sequence-id-498941

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  17. plenti-ZIM3-SpdCas9-2xNLS

    Plasmid
    #248901
    Purpose
    Lentiviral vector expressing human codon-optimized SpdCas9 (deactivated DNAse domain) fused to ZIM3 KRAB domain
    Depositor
    Michael Aregger
    Insert
    ZIM3-FLAG-SpdCas9-2xNLS-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/H840A
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248901-plasmid-map-sequence-id-498950

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  25. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid
    #191099
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Publication
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet-I
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    optimized to human codon usage
    Promoter
    UbC
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191099-plasmid-map-sequence-id-380113

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  34. pCSD_FRB-TALETS3-3xFLAG-2xNLS

    Plasmid
    #107306
    Purpose
    Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    TALE_VEGFA-TS3
    Use
    CRISPR
    Tags
    3x Flag, 2xNLS and FRB
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107306-plasmid-map-sequence-id-204787

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  36. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid
    #139995
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    HF1=N497A/R661A/Q695A/Q926A
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139995-plasmid-map-sequence-id-275425

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  40. pcDNA3.1(+)-AKAP95-FRB

    Plasmid
    #138254
    Purpose
    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Publication
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AKAP95-FRB (AKAP8 Human)
    Tags
    FRB rapamycin binding domain and HA epitope
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138254-plasmid-map-sequence-id-307498

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Showing: 4121 - 4160 of 18759 results