We narrowed to 18,515 results for: tec

1. pBM13g-sd4U

   Plasmid

   #179689

   Purpose

   Bacterial expression of base editor

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [sd4U] UGI-TALE3-DddA-G1397-N (S1330I + A1341V + N1342S + E1370K + T1380I)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S1330I A1341V N1342S E1370K T1380I

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 1046E=dgRNAs[traB,bTub]

   Plasmid

   #149425

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   U6.3-gRNAs[TraB, bTub]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6.3

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

   Plasmid

   #170768

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XhoI
   M.MunI
   M.Ocy1ORF8430P
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261

   Plasmid

   #171434

   Purpose

   Expression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   tdMCP2-GyG-(GGS)X6-HaloTag C261
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lentiguide-Blast-eNMU710

   Plasmid

   #172658

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-eNMUA/pegRNA-eNMUB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDB4961

   Plasmid

   #174015

   Purpose

   pHIS3H plasmid expressing 13Myc-Vps15 from 41nmt1 promoter

   Depositor

   Li-Lin Du

   Article

   Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

   Insert

   Vps15 (ppk19 Fission Yeast)
   

   Use

   Tags

   13Myc, Vps15

   Expression

   Yeast

   Mutation

   Promoter

   41nmt1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB-HD252

   Plasmid

   #172656

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD252A/pegRNA-HD252B
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19-pleCopyCatcher

   Plasmid

   #174061

   Purpose

   CopyCatcher insertion donor for the pale locus

   Depositor

   Ethan Bier

   Article

   Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

   Insert

   Pale (Pale Fly, Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDB4953

   Plasmid

   #174020

   Purpose

   pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter

   Depositor

   Li-Lin Du

   Article

   Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

   Insert

   Atg6
   

   Use

   Tags

   GFP, Atg6

   Expression

   Yeast

   Mutation

   Promoter

   41nmt1

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDB4658

    Plasmid

    #174017

    Purpose

    pDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg8
    

    Use

    Tags

    GFP, Atg8

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDB4958

    Plasmid

    #174014

    Purpose

    pHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg6
    

    Use

    Tags

    CFP, Atg6

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDB4948

    Plasmid

    #174019

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Vps38
    

    Use

    Tags

    Pil1, mCherry, Vps38

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDB4946

    Plasmid

    #174018

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg14 (atg14 Fission Yeast)
    

    Use

    Tags

    Pil1, mCherry, Atg14

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDB4925

    Plasmid

    #174016

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg38(161-190)
    

    Use

    Tags

    Pil1, mCherry, Atg38(161-190)

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET19b-rDAD2 cPGFP(N180)

    Plasmid

    #166001

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(N180)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LSSmOrange- V49A (please see depositor comments)

    Promoter

    T7 promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJIT163-AFID-2

    Plasmid

    #167358

    Purpose

    For inducing predictable deletions in protoplasts and wheat plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3A-Cas9-UDG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLJM1-zeo-2xStrep-ORF68

    Plasmid

    #162659

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLJM1-zeo-2xStrep-UL52

    Plasmid

    #162663

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged UL52 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL52
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO-2xStrep-ORF68 C415A

    Plasmid

    #162633

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 C415A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C415 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-2xStrep-ORF68 H452A

    Plasmid

    #162634

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 H452A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid H452 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA4/TO-2xStrep-ORF68 C79A

    Plasmid

    #162635

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 C79A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C79 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA4/TO-2xStrep-ORF68 K435A

    Plasmid

    #162636

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K435A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid K435 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA4/TO-2xStrep-ORF68 R443A

    Plasmid

    #162637

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 R443A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid R443 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA4/TO-2xStrep-ORF68 K450A/R451A

    Plasmid

    #162638

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K450A/R451A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K450 and R451 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA4/TO-2xStrep-ORF68 R14A

    Plasmid

    #162640

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 R14A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid R14 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA4/TO-2xStrep-ORF68 K310A

    Plasmid

    #162641

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K310A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid K310 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA4/TO-2xStrep-ORF68 R14A/K310A

    Plasmid

    #162642

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 R14A/K310A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids R14 and K310 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4/TO-2xStrep-ORF68 K395A/K396A

    Plasmid

    #162643

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K395A/K396A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K395 and K396 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA4/TO-2xStrep-UL32 C128A

    Plasmid

    #162644

    Purpose

    Expresses N-terminal 2x-Strep-tagged UL32 C128A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C128 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA4/TO-2xStrep-UL32 C502A

    Plasmid

    #162645

    Purpose

    Expresses N-terminal 2x-Strep-tagged UL32 C502A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C502 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA4/TO-2xStrep-UL32 C308A/C309A

    Plasmid

    #162646

    Purpose

    Expresses N-terminal 2x-Strep-tagged UL32 C308A/C309A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids C308 and C309 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA4/TO-2xStrep-UL32 C544A

    Plasmid

    #162647

    Purpose

    Expresses N-terminal 2x-Strep-tagged UL32 C544A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C544 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA4/TO-2xStrep-UL32 H581A

    Plasmid

    #162648

    Purpose

    Expresses N-terminal 2x-Strep-tagged UL32 H581A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid H581 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA4/TO-2xStrep-UL32 C155A

    Plasmid

    #162649

    Purpose

    Expresses N-terminal 2x-Strep-tagged UL32 C155A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C155 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA4/TO-2xStrep-ORF68 C52A

    Plasmid

    #162630

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 C52A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C52 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA4/TO-2xStrep-ORF68 C373A

    Plasmid

    #162631

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 C373A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C373 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA4/TO-2xStrep-ORF68 C191A/C192A

    Plasmid

    #162632

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 C191A/C192A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids C191 and C192 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pWL89A mmPing20F

    Plasmid

    #145789

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20F
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Insertion of the FMV enhancer

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pWL89A mmPing20B

    Plasmid

    #145790

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Insertion of the b-Conglycin Enhancer

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pWL89A NL60

    Plasmid

    #145791

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    NL60
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Insertion of the OCS Enhancer

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pWL89A 2xE

    Plasmid

    #145792

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    2xE
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Insertion of 2 copies of the CMV Enhancer

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pWL89A 4xE

    Plasmid

    #145793

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    4XE
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Insertion of 4 copies of the CMV Enhancer

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRS416-BXG-altTAG

    Plasmid

    #158146

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment (w/ TAG codon)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRS416-BXG

    Plasmid

    #158145

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment (w/ TAG codon)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRS416-BYG

    Plasmid

    #158144

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment (wild-type)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCTCON2-Aga1p-FAPB2.3.6

    Plasmid

    #158142

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCTCON2-Aga1p-FAPB2.3.6L1TAG

    Plasmid

    #158143

    Purpose

    Yeast display reporter for non-display yeast strains (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    FAPB2.3.6L1TAG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. VanR - F165V

    Plasmid

    #158965

    Purpose

    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR - F165V
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type VanR from Caulobacter crescentus with m…

    Promoter

    TEF1

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. VanR - F165R

    Plasmid

    #158966

    Purpose

    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR - F165R
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type VanR from Caulobacter crescentus with m…

    Promoter

    TEF1

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pSWD95

    Plasmid

    #158617

    Purpose

    CckA FRET Sensor

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Xylose

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge