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pINDUCER21-PGBD5

Plasmid

#78121

Purpose

Lentiviral gene expression vector for doxycycline inducible PGBD5 expression

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

PGBD5 (PGBD5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD1 Flag

Plasmid

#74893

Purpose

Express COMMD1 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD1 (COMMD1 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLC-BFP-FADD

Plasmid

#75166

Purpose

LentiCRISPR-BFP with sgRNA targeting human FADD

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

FADD sgRNA (FADD Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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MBD1v1 pcDNA3.1

Plasmid

#78140

Purpose

Mammalian expression of MBD1v1

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD1 (MBD1 Human)

Expression

Mammalian

Mutation

v1 isoform

Promoter

CMV

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV-hygro-PAX9

Plasmid

#75083

Purpose

[Edit] retroviral vector holding human PAX9 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

full length, wild type, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV0-SMAC-HA-eGFP

Plasmid

#67486

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC-HA-eGFP (DIABLO Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
pGEX6P1-human full-length LIC1

Plasmid

#74597

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Flag-MFF-WT

Plasmid

#74379

Purpose

Lentiviral expression vector containing flag tagged WT MFF isoform 5

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pCIG AKT3 E17K

Plasmid

#73050

Purpose

Human AKT3 E17K

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K (AKT3 Human)

Expression

Mammalian

Mutation

E17K

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMK282 (mCherry2-Bsr)

Plasmid

#72798

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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