165,886 results

1. pTriEx-HTNC

   Plasmid

   #13763

   Purpose

   For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

   Depositor

   Klaus Rajewsky

   Article

   Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

   Insert

   His-TAT-NLS-Cre
   

   Use

   Baculovirus

   Tags

   His Tag, NLS, and TAT

   Expression

   Bacterial

   Promoter

   p10 promoter

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. LeGO-G2

   Plasmid

   #25917

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

   Plasmid

   #172620

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a_MYCN_P2A_Hygro_Barcode

   Plasmid

   #120463

   Purpose

   Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYCN (MYCN Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-T2A-moxMaple3

   Plasmid

   #120875

   Purpose

   Mammalian expression of mCherry and moxMaple3

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

   Inserts

   mCherry
   moxMaple3
   

   Expression

   Mammalian

   Mutation

   from mMaple3, moxMaple3 was engineered by replaci…

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKC delta DN

   Plasmid

   #16389

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC delta isoform 2 (Prkcd Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Dominant negative (kinase inactive): K376R

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti HRE-Luc pGK Hygro

   Plasmid

   #118706

   Purpose

   reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   HRE (HIF-responsive promoter (3xHRE)-luc
   

   Use

   Lentiviral

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP‐RNASEH2A

   Plasmid

   #108700

   Purpose

   For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   RNASEH2A (RNASEH2A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3xFLAG-CMV14-GINIP

   Plasmid

   #204845

   Purpose

   Express 3x FLAG tagged-GINIP in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

   Insert

   PHF24 (KIAA1045 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Sept. 14, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pYeDP60-hATP13A2-WT

    Plasmid

    #171377

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Tags

    BAD tag

    Expression

    Yeast

    Promoter

    URA3

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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