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We narrowed to 5,762 results for: Chia

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Showing: 3121 - 3150 of 5762 results

  3. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    cyt-GlycoID-V5
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184640-plasmid-map-sequence-id-366751

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  7. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid
    #195741
    Purpose
    Base editing for making a pre-stop codon in mmoX using phenol inducible system
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195741-plasmid-map-sequence-id-386423

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  10. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid
    #225139
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 protein fused to silaffin-R5 and sfGFP
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225139-plasmid-map-sequence-id-460505

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  13. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    nuc-GlycoID-HA
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  18. p168-759

    Plasmid
    #62619
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62619-plasmid-map-sequence-id-103510

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  30. pRCPam

    Plasmid
    #195861
    Purpose
    Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Insert
    Amber Chromogenic Protein on pRCPam plasmid
    Expression
    Bacterial
    Mutation
    Gln62X
    Available Since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195861-plasmid-map-sequence-id-384172

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Showing: 3121 - 3150 of 5762 results