We narrowed to 4,857 results for: Mela

1. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

   Plasmid

   #159676

   Purpose

   Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEN35-CDKN2A-Ex2-L

   Plasmid

   #110736

   Purpose

   Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   CDKN2A gRNA (CDKN2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT-EGFP-Actin5c

   Plasmid

   #15312

   Depositor

   Ron Vale

   Article

   Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

   Insert

   Actin5c (Act5C Fly)
   

   Use

   Tags

   EGFP and His

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pHIV-ARF-mOrange2

   Plasmid

   #110731

   Purpose

   Mammalian Expression of p14ARF IRES H2B-mOrange2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   p14ARF IRES H2B-mOrange2 (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDF1-WT_RASA2-EF1-Puro

   Plasmid

   #114096

   Purpose

   Express RASA2 in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

   Insert

   RASA2 (RASA2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti_CRISPRi_sgAR2

   Plasmid

   #167001

   Purpose

   Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   AR Promoter sgRNA 2 (AR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti_CRISPRi_sgAR1

   Plasmid

   #167000

   Purpose

   Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   AR Promoter sgRNA 1 (AR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KHBD00332

   Plasmid

   #34455

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG1765 (EcR Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-AR-R405A

   Plasmid

   #126052

   Purpose

   Expresses human androgen receptor (R405A) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

   Insert

   AR (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R405A

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti_CRISPRi_sgAR3

    Plasmid

    #167002

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 3 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJC-elav1.8kb-TALE1-VP64-P10

    Plasmid

    #104609

    Purpose

    Expresses TALE1 under the control of a 1.8kb elav enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.8kb elav enhancer element (elav Fly)
    

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Moe-pDONR221

    Plasmid

    #124239

    Purpose

    Gateway entry clone of Drosophila Moesin cDNA encoding the D isoform.

    Depositor

    Peter Vilmos

    Article

    Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

    Insert

    Drosophila Moesin (Moe Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-AR-R405S

    Plasmid

    #126051

    Purpose

    Expresses human androgen receptor (R405S) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

    Insert

    AR (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R405S

    Promoter

    CMV

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFOSGE_COF4C03

    Plasmid

    #70961

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    His2Av (His2Av Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

15. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid

    #159677

    Purpose

    Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid

    #89890

    Purpose

    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    HA-BirA-2A-citrine
    

    Use

    Unspecified

    Tags

    Citrine

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KHBD00202

    Plasmid

    #34353

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG33261 (Trl Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pCDF1-GRM3 (S610L)

    Plasmid

    #31800

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed serine 610 to leucine

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCDF1-GRM3 (E870K)

    Plasmid

    #31802

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glutamic acid 870 to lysine

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

20. KHBD00020

    Plasmid

    #34182

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1429 (Mef2 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pCDF1-GRM3 (E767K)

    Plasmid

    #31801

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glutamic acid 767 to lysine

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pCDF1-GRM3 (G561E)

    Plasmid

    #31799

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glycine 561 to glutamic acid

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pCDF1-S400F_RASA2-EF1-Puro

    Plasmid

    #114098

    Purpose

    Express mutant RASA2 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

    Insert

    RASA2 (RASA2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S400F

    Promoter

    CMV

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. KHBD00210

    Plasmid

    #34361

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17894 (cnc Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

25. KHBD00009

    Plasmid

    #34172

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5058 (grh Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

26. KHBD00624

    Plasmid

    #39696

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG7450 (CrebA Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

27. KHBD00054

    Plasmid

    #34216

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG2275 (Jra Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pEGFP-N1 Btsz2

    Plasmid

    #40068

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Btsz2 (btsz Fly)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pLentiCRISPRpuro-sgSEC24D

    Plasmid

    #231565

    Purpose

    pLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24D (Puromycin selection marker)

    Depositor

    Rizwan Haq

    Article

    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

    Insert

    sgSEC24D (SEC24D Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF-1a

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMVP-SEC24C

    Plasmid

    #231559

    Purpose

    pMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)

    Depositor

    Rizwan Haq

    Article

    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

    Insert

    SEC24C (SEC24C Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    silent PAM mutations (G67 (GGG>GGA) and A115 (…

    Promoter

    CMV

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. K365-iLid

    Plasmid

    #188456

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Zvonimir Dogic

    Article

    Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

    Insert

    Kinesin 1-365 (Khc Fly)
    

    Use

    Tags

    6xHis and iLid

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-365

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. K365-micro

    Plasmid

    #188457

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Zvonimir Dogic

    Article

    Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

    Insert

    Kinesin 1-365 (Khc Fly)
    

    Use

    Tags

    6xHis-MBP-TEV and SspB micro

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-365

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD

    Plasmid

    #148514

    Purpose

    Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD

    Plasmid

    #148515

    Purpose

    Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-675

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    DmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAc5.1B-EGFP-DmNot1_1-1717_X

    Plasmid

    #147982

    Purpose

    Insect Expression of DmNot1_1-1717

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1-1717 (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    6 silent mutations and two non silent N605S, K759…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc5.1B-EGFP-DmNot1_1-1717-K1537SG1562EQ1658A_X

    Plasmid

    #147986

    Purpose

    Insect Expression of DmNot1_1-1717-K1537SG1562EQ1658A

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1-1717-K1537SG1562EQ1658A (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    6 silent mutations and two non silent N605S, K759…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAc5.1B-EGFP-DmNot1_1-1717-R1197AN1203AF1204A_X

    Plasmid

    #147987

    Purpose

    Insect Expression of DmNot1_1-1717-R1197AN1203AF1204A

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1-1717-R1197AN1203AF1204A (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    6 silent mutations and two non silent N605S, K759…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAc5.1B-EGFP-DmNot1_1-1717-RNFKGQtoAAASEA_X

    Plasmid

    #147988

    Purpose

    Insect Expression of DmNot1_1-1717-RNFKGQtoAAASEA

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1-1717-RNFKGQtoAAASEA (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    6 silent mutations and two non silent N605S, K759…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1B-EGFP-DmNot1_1-1945-dsRNAres_X

    Plasmid

    #147989

    Purpose

    Insect Expression of DmNot1_1-1945-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1-1945-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    8 silent mutations and two non silent N605S, K759…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1B-lambdaN-HA-DmNot1-G1562E-dsRNAres_X

    Plasmid

    #147996

    Purpose

    Insect Expression of DmNot1-G1562E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1-G1562E-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge