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CMV-SauriABE8e

Plasmid

#189927

Purpose

Plasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SauriABE8e

Expression

Mammalian

Mutation

Nme2Cas9 D16A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag3B-ERT2CCre

Plasmid

#106371

Purpose

C-terminal part of the split-CreERT2 System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009 Dec 16;4(12):e8354. doi:

Insert

ERT2CCre

Use

Cre/Lox

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD2

Plasmid

#68161

Purpose

Universal domesticator plasmid for level 0 parts

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Plasmid

#207861

Purpose

AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med25

Plasmid

#64771

Purpose

Mammalian protein expression of flag-tagged Med25

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25 (MED25 Human)

Tags

Flag

Expression

Mammalian

Promoter

pCMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pBAD/HisB-Medium-FT

Plasmid

#31917

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Tags

His

Expression

Bacterial

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

araB

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pHLUM (version 2)

Plasmid

#64166

Purpose

harbours marker genes HIS3, LEU2, URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAcGHLT-B-DDB1

Plasmid

#48638

Purpose

Expresses Full Lenght DDB1 in Insect Cells (ampR)

Depositor

Ning Zheng

Article

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

DDB1 (DDB1 Human)

Expression

Insect

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
pUCDM

Plasmid

#110742

Purpose

Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Promoter

Polyhedrin and p10 promoters

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Tags

6xHIS and MBP

Expression

Bacterial

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
pSVL(D2m)

Plasmid

#29336

Depositor

John Taylor

Article

Kuo et al J Virol. 1989 May . 63(5):1945-50.

Insert

Dimer of HDV genome with 2 nucleotide deletion in delta antigen

Expression

Mammalian

Mutation

Contains a dimer of the complete HDV genome excep…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pBABE-Flag-Cdk9-IRES-eGFP

Plasmid

#28096

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pComb3HSS

Plasmid

#64133

Purpose

2nd generation plasmid for phage display on modified geneIII, contains stuffer fragment

Depositor

Carlos Barbas

Article

Barbas et al Methods, A companion to Methods in Enzym

Inserts

SS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer

Use

Phage display

Tags

geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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YES1 Y537F

Plasmid

#51299

Purpose

Expression of YES1 Y537F

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral

Mutation

Y537F

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 Y242/287A

Plasmid

#32822

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242/287A

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tipin

Plasmid

#22889

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pUCHR-inLuc

Plasmid

#58955

Purpose

HIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA008

Plasmid

#48817

Purpose

Provides the alcA promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

alcA promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDAC569

Plasmid

#195241

Purpose

Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP

Expression

Mammalian

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

Plasmid

#171380

Purpose

Expression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized RfxCas13d

Tags

HA

Expression

Mammalian

Promoter

CMV, U6

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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