We narrowed to 15,732 results for: LEA

1. pExodus CMV.Trex2

   Plasmid

   #40210

   Purpose

   Mammalian expression of codon-optimized Trex2.

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Insert

   Three prime repair exonuclease 2 (Trex2 Mouse)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. SWEET11:SWEET11-2A-GFP-GUS

   Plasmid

   #172484

   Purpose

   Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

   Depositor

   Wolf Frommer

   Article

   Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

   Insert

   SWEET11 (AT3G48740 Mustard Weed)
   

   Tags

   GFP, GUS

   Expression

   Plant

   Promoter

   SWEET11

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-mCherry-CyrCR1.mutant

   Plasmid

   #128746

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Mutation

   5 point mutations disrupting the miR-7 site

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-Hsp90 M(272-617)

   Plasmid

   #22482

   Purpose

   Please note this plasmid contains GST-Hsp90 residues 273-617.

   Depositor

   William Sessa

   Article

   Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

   Insert

   Hsp90alpha M domain (HSP90AA2P Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mCherry-Rheb(C181S)

   Plasmid

   #192437

   Purpose

   Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-Rheb(C181S) (RHEB Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Rheb Cys 181 mutated to Ser; abolishes C-terminal…

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUW-TetO-tdTomato-cNLS

   Plasmid

   #138532

   Purpose

   Lentiviral inducible expression of tdTomato with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   tdTomato
   

   Use

   Lentiviral

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE-CMV

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TfR-mCh-DHFR

   Plasmid

   #173013

   Purpose

   For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   TfR-mCh-DHFR
   

   Tags

   DHFR and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVL.Active/Repressed TLR (Sce)

   Plasmid

   #45575

   Purpose

   Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   eGFP with I-Sce I TS
   +3 mCherry
   

   Use

   Lentiviral

   Tags

   T2A dislinker and iRFP

   Expression

   Bacterial and Mammalian

   Mutation

   embedded I-Sce I TS from 163-185

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. RHBDD1 pmRFP-N2 (1390)

   Plasmid

   #62049

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   RHBDD1 (RHBDD1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. PB_EF1a-CasRx-msfGFP-2A-Blast

    Plasmid

    #226010

    Purpose

    CasRx protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    CasRx RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUW-TetO-mCherry-cNLS

    Plasmid

    #138531

    Purpose

    Lentiviral inducible expression of mCherry with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. RHBDD1 pEGFP-N2 (1078)

    Plasmid

    #62028

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    RHBDD1 (RHBDD1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-mCherry-LINXa3

    Plasmid

    #73614

    Purpose

    Light-induced nuclear export switch for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    Chicken B-Actin

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. DHFR-GFP-th-NL1

    Plasmid

    #173007

    Purpose

    For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-GFP-NL1
    

    Tags

    DHFR and EGFP

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-AMPKsub-YPet-NES

    Plasmid

    #84635

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. DHFR-GFP-th-GluA1

    Plasmid

    #173008

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-GFP-th-GluA1
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-REPORT(PGK)

    Plasmid

    #172328

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUW-TetO-DsRed-cNLS

    Plasmid

    #138533

    Purpose

    Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    DsRed-Express2
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTriEx-mCherry-LINXa3-nano

    Plasmid

    #73616

    Purpose

    Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3-nano
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Src(WT)-SsrA(Y7I)-mCer3

    Plasmid

    #220998

    Purpose

    This is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminal

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA, mCerulean3 (Src Mouse)
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Y7I in SsrA

    Promoter

    CMV

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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