We narrowed to 11,424 results for: 112

1. AAVS1-Puro CAG CLTX-CAR

   Plasmid

   #157743

   Purpose

   knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   chlorotoxin containing chimeric antigen receptor targeting glioblastoma
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6-His-MBP-TEV-FnCpf1

   Plasmid

   #90094

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 (humanized)
   

   Use

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

   Plasmid

   #211817

   Purpose

   AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   N-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter and U6 promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

   Plasmid

   #211818

   Purpose

   AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   C-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter and U6 promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-GfABC1D-SaCas9-WPRE3-pA

   Plasmid

   #203540

   Purpose

   SaCas9 expression in astrocytes

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   SaCas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-GfABC1D-NLS-GFP-WPRE-synpA-L1-R2

   Plasmid

   #203541

   Purpose

   gRNA expression in astrocytes

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   NLS-GFP, 2x U6-RNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. SSpB-mCherry-p63DH

   Plasmid

   #176112

   Purpose

   Heterodimerization with iLID, RhoA activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   RHOGEF p63 (ARHGEF25 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLIK_neo_3xflag_CHEK2

   Plasmid

   #136536

   Purpose

   Lentiviral expression vector for an inducible 3xflag-tagged CHEK2

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   CHEK2 (CHEK2 Human)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCVhyg-HA-FLAG_Ubc9_WT

   Plasmid

   #164936

   Purpose

   Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   human UBC9 (UBE2I Human)
   

   Use

   Retroviral

   Tags

   FLAG & HA tags

   Expression

   Mutation

   Promoter

   PGK promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221-SLC10A5_STOP

    Plasmid

    #161121

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC10A5 (SLC10A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pInducer10-SAE1_sh1

    Plasmid

    #168984

    Purpose

    Expresses RFP cDNA and SAE1 shRNA 1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FR_HNF4A2-C106R

    Plasmid

    #31123

    Depositor

    Gerhart Ryffel

    Article

    Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 2, mutation C106R. Wild type seq…

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-FLAG-SRSF3_Truncated_Codon.Optimized

    Plasmid

    #172346

    Purpose

    Expression of FLAG-tagged, codon-optimized, truncated SRSF3

    Depositor

    Sujatha Jagannathan

    Article

    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

    Insert

    serine and arginine rich splicing factor 3 (SRSF3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized, truncated at amino acid 114 with…

    Promoter

    EF-1a

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. RGFR2 X055_pECIA2

    Plasmid

    #115112

    Purpose

    Bait vector RGFR2 X055_pECIA2 should be used with prey vector RGFR2 X055_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G48940 (AT5G48940 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. GRM7-DuET

    Plasmid

    #213305

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM7 (GRM7 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA

    Plasmid

    #211819

    Purpose

    lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9, tracrRNA and blasticidin resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a promoter and miniU6 promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

    Plasmid

    #211821

    Purpose

    PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter, U6 promoter and hPGK pr…

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR

    Plasmid

    #211815

    Purpose

    lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9, tracrRNA and puromycin selection gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a promoter, U6 promoter, and hPGK promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF1791

    Plasmid

    #144112

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXE3 (FOXE3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PGK EGFP-LC3B

    Plasmid

    #200427

    Purpose

    Mammalian expression of fluorescently-tagged marker for autophagic vesicles

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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