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We narrowed to 5,293 results for: pcDNA3.1+

Showing: 2101 - 2120 of 5293 results

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pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

Plasmid

#49063

Purpose

Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Article

Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

Insert

human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)

Use

Tags

3x Flag and mVenus

Expression

Mammalian

Mutation

2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …

Promoter

CMV

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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XE252 pCDNA3.1(zeo)-hDsh3-333-716

Plasmid

#16757

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-333-716-Dsh3 (DVL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 333-716

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X

Plasmid

#16759

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

VSV-KLHL12-Q405X (KLHL12 Human)

Use

Tags

vsv

Expression

Mammalian

Mutation

Q405STOP

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG

Plasmid

#29583

Depositor

Anna Huttenlocher

Article

Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

Insert

phosphatidylinositol phosphate kinase type I gamma

Use

Tags

Flag and GFP

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Feb. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only

Plasmid

#69907

Purpose

Expresses a circular RNA containing the 5' half of GFP in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

GFP, ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

Plasmid

#111459

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

Ha Tag

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523), C643A

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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(363) pcDNA3.1-Nup62-Ubc-Flag-EPEA

Plasmid

#168098

Purpose

Nucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tag

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

Nup62-Ubc-Flag-EPEA

Use

Tags

EPEA-tag, Flag-tag, and Ubc-Tag

Expression

Mammalian

Mutation

P229A, S283T on Nup62 native sequence

Promoter

T7/cmv

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

Plasmid

#169992

Purpose

Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

TEV-siriusGFP-FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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XE254 pCDNA3.1(zeo)-hDsh3-497-716

Plasmid

#16755

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-497-716-Dsh3 (DVL3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

aa 497-716

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG mTOR 1968-2549 S2035T

Plasmid

#23000

Depositor

Jing Huang

Article

Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

mTOR (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S2035T aa 1968-2549 only

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-847

Plasmid

#69894

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

Plasmid

#62978

Purpose

Expresses myc-tagged mouse βTrCP residues 292-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

codes for residues 292-569

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

Plasmid

#166796

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

KK93EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

Plasmid

#166800

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

E597R

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

Plasmid

#49064

Purpose

Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Article

Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

Insert

human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)

Use

Tags

3x Flag and mVenus

Expression

Mammalian

Mutation

2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …

Promoter

CMV

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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p5064 pcDNA3.1/V5-His-TOPO hBrd4 (1-461)

Plasmid

#14443

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-461) (BRD4 Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 1-461

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)

Plasmid

#86818

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69896

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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