We narrowed to 7,013 results for: tet

1. pCS-TRE-FHDnd1-Ubc-rtTA-I2G

   Plasmid

   #70076

   Purpose

   2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Silent mutations are introduced to escape from RN…

   Promoter

   Tet responsible promoter

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

   Plasmid

   #70075

   Purpose

   2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Y102A. Silent mutations are introduced to escape …

   Promoter

   Tet responsible promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIX-hN1ICD

   Plasmid

   #91897

   Purpose

   Doxycycline inducible expression of human Notch1 ICD

   Depositor

   Julien Sage

   Insert

   Notch1 intracellular domain (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codons 1770 to 2555 of human NOTCH1

   Promoter

   TRE promoter, Tet ON

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector

   Plasmid

   #73707

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   T231A/S232A

   Promoter

   CMV/tet

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

5. HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

   Plasmid

   #73702

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   448-AAAA-451

   Promoter

   CMV/tet

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pRMC2

   Plasmid

   #68940

   Purpose

   tetracycline-inducible expression vector for Staphylococcus aureus

   Depositor

   Tim Foster

   Article

   Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

   Type

   Empty backbone

   Use

   Tet inducible e. coli/staphylococcus shuttle vect…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. iF59 PB-TO-oNgn2-puro

   Plasmid

   #204734

   Purpose

   Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Optimized neurogenin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. iC13 PB-TO-oNgn2-BFP-puro

   Plasmid

   #204733

   Purpose

   Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Optimized neurogenin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRT-TODestGFP_RNAseH1

   Plasmid

   #65784

   Purpose

   expresses GFP-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Zebrafish, Human)
   

   Use

   Tags

   GFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT-TODestRFP_RNAseH1

    Plasmid

    #65785

    Purpose

    expresses RFP-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Zebrafish, Human)
    

    Use

    Tags

    RFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRT-TODestFLAGHA_RNAseH1

    Plasmid

    #65782

    Purpose

    expresses FLAGHA-tagged RNAsH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Zebrafish, Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT-DestFLAGHA_RNAseH1

    Plasmid

    #65783

    Purpose

    constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Zebrafish, Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector

    Plasmid

    #73706

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    448-AAAA-451 and D143N

    Promoter

    CMV/tet

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pTS35.41

    Plasmid

    #106263

    Purpose

    Polycistronic expression of luc, ekivR using P2A

    Depositor

    Vera Meyer

    Article

    Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

    Insert

    luc-P2A-ekivR
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Tet-On

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT-TODestRFP_HuC

    Plasmid

    #65765

    Purpose

    expresses RFP-tagged HuC in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

16. PB-TO-MYOD1-shOct4

    Plasmid

    #182309

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    shOct4

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBSJL1

    Plasmid

    #71261

    Purpose

    E. coli-Veillonella shuttle plasmid

    Depositor

    Felicia Qi

    Article

    Liu et al Appl Environ Microbiol. 2012 May;78(9):3

    Type

    Empty backbone

    Use

    E. coli-veillonella shuttle plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

18. AAVS1-SA-2A-NEO-CAG-RTTA3

    Plasmid

    #60431

    Purpose

    neomycin resistance; constitutive promoter driving reverse tet activator

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    rtTA3
    

    Use

    Zfn donor

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT-TODestRFP_HuD

    Plasmid

    #65766

    Purpose

    expresses RFP-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V23M mutation in RFP

    Promoter

    CMV, 2x Tet

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFRT-TODestGFP_HuD

    Plasmid

    #65763

    Purpose

    expresses GFP-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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