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Showing: 10631 - 10640 of 168638 results

  5. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid
    #176836
    Purpose
    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-
    Insert
    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    Use
    Unspecified
    Mutation
    See Depositor Comments Below
    Promoter
    Tet operator (tetracycline inducible expression)
    Available Since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176836-plasmid-map-sequence-id-346773

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  6. pGDP1 fosA

    Plasmid
    #112889
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    fosA
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    Pbla
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112889-plasmid-map-sequence-id-219899

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  7. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139996-plasmid-map-sequence-id-275405

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  9. AAVS1-iPE-Plus

    Plasmid
    #214062
    Purpose
    inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus
    Depositor
    Ting Zhou
    Article
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Insert
    PE-Plus
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available Since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214062-plasmid-map-sequence-id-429371

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Showing: 10631 - 10640 of 168638 results